GbE对TCE诱导人KC细胞氧化损伤的影响  

Protective effects of extracts from leaves of Ginkgo biloba on TCE-induced oxidation insult in human keratinocytes

作　　者：涂登云[1] 沈彤[1] 丁锐[1] 魏凌珍[1] 孙美芳[1] 朱启星[1] 

机构地区：[1]安徽医科大学公共卫生学院毒理中心实验室,合肥230032

出　　处：《中国公共卫生》2005年第7期802-804,共3页Chinese Journal of Public Health

基　　金：安徽省自然科学基金项目(03043810);安徽省教育厅自然科学重点科研项目(2003KJ036ZD)

摘　　要：目的探讨三氯乙烯(Trichloroethylene,TCE)对人角质形成细胞(Keratinocyte,KC)氧化损伤的影响及银杏叶提取物(ExtractsfromtheLeavesofGinkgobiloba,GbE)对其拮抗作用。方法采用体外KC无血清培养方法,用中性红吸收实验(NetrualRedUptake,NRU)测定TCE对KC细胞毒性的半数抑制浓度NR50;用乳酸脱氢酶(LDH)法检测KC的损伤;用硫代巴比妥酸法测定KC细胞脂质过氧化物丙二醛(MDA)活力;同时检测细胞内抗氧化酶超氧化物歧化酶(SOD)的活力。结果TCE对KC的NR50为4.53mmol/L;KC在TCE(0.125,0.25,0.5,1.0,2.0mmol/L)作用1,2,3,4h后,LDH释放增加,且具有时间和剂量依赖性,KC在TCE作用4h后,MDA的生成量显著减少,且呈浓度依赖性,与溶剂对照组相比,P<0.05;GbE(10,50,100,150,200mol/L)可以浓度依赖性的减少LDH释放、MDA的生成量,增加SOD的生成量。结论GbE可拮抗TCE引起的人KC损伤。Objective To further assess the effects of extracts from the leaves of Ginkgo biloba on Trichloroethylene (TCE)-induced normal human epidermal keratinocytes (NHEK) oxidation insult.Methods Cytotoxicity was determined by netrual red uptake (NRU) and lactate dehydrogenase release (LDH) test,lipid peroxidation of cells was evaluated by malondialdehyde (MDA) and superoxide dismutase (SOD),respectively.Results Cytotoxicity asays yielded the concentration producing 50% reduction of Netrual Red Uptake (NR_(50)) for TCE was 4.53?mmol/L.Compared with control,treatment of with various concentration of TCE caused a great decrease in cell viability (P<0.05).A concentration-and time -dependent release of LDH and a concentration dependent increase of MDA levels were observed.In contrast,SOD activity was inhibited by concentration dependent mode.Ginkgo biloba 10～200?mg/L dose-dependently attenuated the cytotoxic effect of TCE.Ginkgo biloba also inhibited TCE (0.5?mmol/L)-induced LDH release,elevation of lipide peroxidation and decline of antioxidant enzyme activities.Conclusion Ginkgo biloba protected NHEK from TCE-induced cytotoxicity.

关 键 词：三氯乙烯 角质形成细胞 银杏叶提取物(GbE) 脂质过氧化 

分 类 号：R75[医药卫生—皮肤病学与性病学] R446.113[医药卫生—临床医学]