吸入二硫化碳对小鼠生殖细胞损伤的影响  

Study on damage of germ cells induced by inhalation carbon disulfide in mice

作　　者：吴磊[1] 谭晓东[1] 

机构地区：[1]武汉大学公共卫生学院卫生学教研室,武汉430071

出　　处：《中国公共卫生》2005年第7期833-834,共2页Chinese Journal of Public Health

摘　　要：目的观察二硫化碳吸入对小鼠生殖细胞的损伤。方法以浓度为0,200,400,800mg/m3的二硫化碳蒸气给小鼠做静式吸入染毒5周,每天2h。用单细胞凝胶电泳实验和流式细胞术检测二硫化碳对精子细胞的损伤。结果低、中、高染毒组与对照组小鼠精子细胞彗星的拖尾率分别为48.50%,71.00%,86.50%和23.50%,经χ2检验各试验组与对照组相比差异有统计学意义(P<0.01);彗星头部百分比分别为(62.9949±8.6242)%,(56.6580±10.5990)%,(36.5432±12.0002)%和(86.8926±5.0219)%,各试验组与对照组彗星头部百分比差异有统计学意义,并且各个试验组之间差异也有统计学意义(P<0.001);尾长分别为(8.6812±4.333),(14.0392±4.168),(28.6601±8.369)和(5.9913±1.924)μm,中、高剂量组与对照组之间相比差异有统计学意义。试验组与对照组生殖细胞的凋亡率分别为12.53%,14.93%,29.09%和11.97%,其中高剂量组与对照组之间相比差异有统计学意义。结论亚慢性吸入二硫化碳对小鼠精子细胞DNA产生损伤。Objective To study the damage effect of inhalation carbon disulfide on germ cells of mice.Methods The damage of germ cells of mice exposed to carbon disulfide by 2?h static inhalation per day at different concentrations(0,200,400,800?mg/m^3)for 5 weeks was analyzed with single cell gel electrophoresis(SCGE)and flow cytometry(FCM).Results In four groups,the rate of DNA damage were 48.50%,71.00%,86.50% and 23.50% respectively,there was significant difference between the experiment group and control(P<0.01);the percentage of head of the coment were (62.9949±8.6242)%,(56.6580±10.5990)%,(36.5432.0002)% and (86.8926±5.0219)% respectively;there was significant difference between the experiment group and control and every experiment groups(P<0.001);the tail length in every groups were (8.6812±4.333),(14.0392±4.168),(28.6601±8.369) and (5.9913±1.924)?μm respectively,there was significant difference between middle,high dosage groups and control(P<0.001).The rate of apoptosis in all groups were 12.53%,14.93%,29.09% and 11.97% respectively,and only high dosage group had significant difference compared with the control.Conclusion The sub-chronic carbon disulfide exposed can induce DNA damage of germ cells.

关 键 词：二硫化碳 单细胞凝胶电泳实验 流式细胞术 精子 

分 类 号：R135.14[医药卫生—劳动卫生]