软骨细胞植入胶原蛋白重建类软骨组织的体外培养研究  

Culture of cartilaginoid tissue reconstitued by chondro-cytes embedded within collagen gels

作　　者：沈强[1] 臧鸿声[1] 贾连顺[1] 董荣春[1] 李家顺[1] 赵定麟[1] 

机构地区：[1]第二军医大学长征医院骨科,第二军医大学病理学教研室

出　　处：《中华外科杂志》1994年第4期252-254,T034,共4页Chinese Journal of Surgery

摘　　要：本实验采用鸡胚胎第３８期胸软骨，经酶消化法分离软骨细胞，均匀弥散植入外源性胶原蛋白交联形成的凝胶体内。经过体外培养８天，软骨细胞数目增加约３倍。组织学观察显示：软骨细胞均匀分布于胶原蛋白三维网络之间，细胞分化良好，细胞周围可见类似正常软骨组织之陷窝（Ｌａｃｕｎａ）。胶原网络间有蛋白多糖蓄积现象。组织化学分析提示软骨细胞合成分泌Ⅱ１型胶原蛋白。实验结果提示：三维胶原蛋白凝胶构成类似软骨组织的细胞外生存环境，有利于软骨细胞生长、增殖与分化，并使其向成软骨组织方向发展，形成软骨样组织。AbstractChondrocytes from the sterna of embrvonal chick-en were dissociated by enzymatic digestion. Dissociatedcells were cultured within collagen gels for 8 days.Thecells increased 3 fold in 8 days. The gels gradually be-came white cartilaginoid tissue in appeareace. Morpho-logic study showed that cells lied in lacunae and main-tained the differentiated characteristics of cartilage.Extracellular matrix produced by cells deposited on ex-ogenous collagen. Biochemical analysis showed thatthe collagen newly synthesised by the cells was type Ⅱcollagen. This experiment demonstrates that collagengels provide a suitable environment for chondrocvtegrowth, proliferation and differentiation,and chondro-cytes embedded within collagen gels reconstitute carti-laginoid tissue.

关 键 词：软骨细胞 胶原蛋白 体外培养 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学]