非水毛细管电泳拆分抗胆碱能药物对映体  

Chiral Separation System of Anticholinergic Drugs in Nonaqueous Capillary Electrophoresis

作　　者：陶胜源[1] 杜国华[2] 谢剑炜[2] 

机构地区：[1]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071 [2]中国人民解放军军事医学科学院毒物药物研究所,北京100850

出　　处：《解放军药学学报》2005年第3期206-208,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立非水毛细管电泳拆分体系拆分抗胆碱能药物。方法以有机溶剂甲醇为分离介质,磷酸和氢氧化钠为支持电解质,采用带负电荷的环糊精作手性选择剂,对几种抗胆碱能药物进行了毛细管电泳拆分,并探讨了影响拆分的因素。结果以带负电荷的环糊精HDMSβCD为手性添加剂,在含有20mmol·L-1H3PO4和10mmol·L-1NaOH的甲醇介质中,成功分离了6种抗胆碱能手性药物。结论该方法具有操作简单、重现性好、分析时间短等优点,值得推广应用。Aim To establish a chiral separation system in nonaqueous capillary electrophoresis to separate anticholinergic drugs.Methods A non-aqueous capillary electrophoresis (NACE) with heptakis(2,3-di-O-methyl-6-O-sulfato)-β-cyclodextrin as chiral selector for separation anticholinergic drug enantiomers was described.Phosphoric acid and sodium hydroxide medium with methanol as solvent was used for background electrolyte (BGE).Optimal separation conditions were investigated.Results Enantioseparation were achieved in 20mmol·L^(-1) H3PO4 and 10mmol·L^(-1) NaOH dissolved in acidic methanolic BGEs when the HDMS-β-CD concentration was at 9mmol·L^(-1).Conclusion The NACE method provided greater separation efficiency and achieved baseline resolution for all six basic anticholinergic drugs.

关 键 词：非水毛细管电泳 抗胆碱能药物 手性拆分 

分 类 号：R927.11[医药卫生—药学]