涵水胶囊中淫羊藿苷的含量测定  

Determination of Icariin in Hanshui Capsule by HPLC

作　　者：耿晖[1] 王梅[1] 王爱武[1] 吴丽敏[1] 田景奎[1] 姚逢楠[1] 

机构地区：[1]山东省立医院天然药物研究中心,山东济南250021

出　　处：《解放军药学学报》2005年第3期231-233,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立涵水胶囊中淫羊藿苷含量测定方法。方法高效液相色谱法,AlltimaC18柱(250mm×4.6mm,5μm),流动相:乙腈-水(25.4∶74.6)。柱温:30℃,流速:1ml/min,检测波长:270nm。结果淫羊藿苷在0.1208～0.604g范围内线性关系良好(r=0.9999),回收率101.3%,RSD=2.2%(n=5)。结论本方法简便、灵敏准确、重复性好,可用于控制涵水胶囊的质量。Aim To establish an HPLC method for determination of Icariin in Hanshui Capsule.Methods The Alltima C(18) column(250mm×4.6mm,5μm) was used.The mobile phase was Acetonitrile-water(25.4∶74.6).The flow rate was 1ml/min.The column temperature was 30℃.Detective wavelength was 270nm.Results The linear range of Icariin was 0.120 8～0.604μg,r=0.999 9.The average recovery was 101.3%,RSD=2.2%(n=5).Conclusion The method was accurate and sensitive for the determination of Icariin in Hanshui Capsule.

关 键 词：涵水胶囊 HPLC 淫羊藿苷 含量测定 

分 类 号：R927.2[医药卫生—药学]