鼎湖山厚壳桂Cryptocarya chinensis不同年龄级种群遗传多样性  

Genetic Diversity of Cryptocarya Chinensis Life Stages in Dinghu Mountain,China

作　　者：高三红[1] 王峥峰[2] 张军丽[1] 田胜尼[2] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]中国科学院华南植物园广东省数字植物园重点实验室,广东广州510650

出　　处：《中山大学学报（自然科学版）》2005年第B06期209-212,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金(30300055);广东省自然科学基金(031264);瑞典IFS基金(D/3239-1);中国科学院华南植物园所长基金资助项目

摘　　要：采用ISSR分子标记技术,对鼎湖山常绿阔叶林中的演替顶极种厚壳桂不同年龄级的遗传多样性进行了研究。同其它生活史特性相似的物种相比较,鼎湖山厚壳桂种群的遗传多样性水平偏低(HT=0.1090)。3个不同年龄级———成体、小树、幼苗的遗传多样性(He)分别为0.0759、0.0956和0.1265。幼苗的遗传多样性指数高于成体及小树。3个不同年龄级种群之间的遗传分化Gst和φst(AMOVA)分别为0.0890和0.0644(P<0.0002),表明厚壳桂年龄级之间遗传分化较小,但分化显著。成体、小树、幼苗之间,其遗传距离随着年龄级逐渐加大。鼎湖山厚壳桂低的遗传多样性是由于缺乏不同地区种群间基因流造成的,但世代间遗传分化可能由取样误差所产生。Genetic diversity and population structure of Cryptocarya Chinensis were examined in three different life stages (adults, juveniles, seedlings) in Dinghu mountain using ISSR markers. The results indicated the level of genetic diversity of C. chinensis(H_e=0\^1090) was relatively low compared with those species with similar life history, and the degree of genetic differentiation between life stages (G_(st)=0\^0890,_(st)=0\^064 4) was low. Seedlings had higher genetic diversity than adults and juveniles. We also found the increase in genetic distance from adults to seedlings. Compared with the similar life history species, C.chinensis has relatively low genetic diversity, which may be caused by low gene flow between populations. However, genetic differentiation might be attributed to sampling deviation.

关 键 词：ISSR 厚壳桂 遗传多样性 年龄级 

分 类 号：Q948[生物学—植物学]