炎性介质在肢体缺血再灌注时的表达和临床意义  

Expression of inflammation mediators in the reperfusion of the ischemic limb

作　　者：张忠[1] 陈瑾[1] 姚菊[1] 沈施仁[1] 

机构地区：[1]南通大学附属医院麻醉科,221001

出　　处：《江苏医药》2005年第7期515-516,共2页Jiangsu Medical Journal

摘　　要：目的探讨肢体缺血再灌注时炎性介质的反应。方法21例择期手术患者随机分非缺血对照组和缺血再灌注组。用放射免疫法、硫代巴比妥法、ELISA法、硝酸还原酶法分别测定TXB2、LPO、IL-8、NO的值。结果肢体缺血再灌注后10min,LPO显著升高。再灌注后18minTXB2、TXB2/6RetoPGF12的比值、LPO、IL-8显著升高,NO显著降低。结论炎性介质TXB2、LPO、IL8、NO参与肢体缺血再灌注损伤,是属于一种炎症反应。Objective To observe the effects of inflammation mediators in the reperfusion of ischemic limb.Methods 21 patients selected for limb operation were divided randomly into two groups:nonischemic control and ischemic reperfusion.The values of TXB_2,Lipid peroxidation(LPO),interleukin-8(IL-8) and nitric oxide (NO)were measured by radioimmunoassay,sulfobarbifurate assay, ELISA and nitrate reductase assay respectively.Results The LPO concentration elevated markedly in ischemic limb of reperfusion group after 10 min.The levels of TXB_2,TXB_2/6-keto- PGFla, LPO,IL-8 were significantly increased after 18 min.The level of NO decreased markedly. Conclusions TXB_2,LPO,IL-8,NO may be implicated in the damage of reperfusion of ischemic limb.The injury during reperfusion of ischemic limb is a kind of imflammatoy response.

关 键 词：炎性介质 临床意义 肢体缺血再灌注损伤 TXB2 择期手术患者 ELISA法 硝酸还原酶法 IL-8 放射免疫法 硫代巴比妥 LPO 炎症反应 对照组 升高 

分 类 号：R562.25[医药卫生—呼吸系统] R654.3[医药卫生—内科学]