人肝癌细胞内多种元素分布特征的分子活化研究  

Studies on cellular distribution of elements in human hepatocellular carcinoma samples by molecular activation analysis

作　　者：邓贵龙[1] 陈春英[1] 章佩群[1] 刘颖斌[2] 赵九江[1] 王建伟[2] 许斌[2] 彭淑牖[2] 柴之芳[1] 

机构地区：[1]中国科学院高能物理研究所核分析技术重点实验室,北京100049 [2]浙江大学医学院附属第二医院普外科,杭州310039

出　　处：《核技术》2005年第7期513-519,共7页Nuclear Techniques

基　　金：国家自然科学面上基金(No.20071032);十五重大项目(No.10490180);中国科学院重要方向性项目(No.KJCX2-N101)资助

摘　　要：为探讨微量元素在原发性肝癌亚细胞组分中的分布及在肝癌发生发展中的变化,采用分子活化分析方法测定了肝癌和正常肝脏细胞亚细胞组分中的17种不同宏量和微量元素的分布,t检验比较两者之间的元素含量的差别。结果发现Br、Ca、Cd和Cs在肝癌各亚细胞组分中的含量明显高于正常人,大部分具有统计学显著性差异。肝癌微粒体Fe明显低于正常人肝,为正常人的一半(P<0.05),而其它亚细胞组分均高于正常人肝。La和Ce的分布与Fe类似。Sb和Zn在肝癌细胞核中的含量则明显低于正常人肝(P<0.05、P<0.05)。K、Na在肝癌胞浆的含量明显高于正常人(P<0.05)。Ba和Rb在肝癌和正常肝脏的分布没有显著性差异。本文还探讨了Fe、Cd和K等元素含量的变化与肝癌发生的关系。肝癌与正常肝脏的亚细胞组分之间,多数微量元素的空间分布有着显著不同,提示微量元素在肝癌发生发展中起着重要作用。The distribution patterns of 17 elements in the subcellular fractions of nuclei, mitochondria, lysosome, microsome and cytosol of human hepatocellular carcinoma (HCC) and normal liver samples were investigated by using molecular activation analysis (MAA) and differential centrifugation. Their significant difference was checked by the Student's t-test. These elements exhibit inhomogeneous distributions in each subcellular fraction. Some elements have no significant difference between hepatocellular carcinoma and normal liver samples. However, the concentrations of Br, Ca, Cd and Cs are significantly higher in each component of hepatocarcinoma than in normal liver. The content of Fe in microsome of HCC is significantly lower, almost half of normal liver samples, but higher in other subcellular fractions than in those of normal tissues. The rare earth elements of La and Ce have the patterns similar to Fe. The concentrations of Sb and Zn in nuclei of HCC are obviously lower (P less than or equal 0.05, P less than or equal 0.05). The contents of K and Na are higher in cytosol of HCC (P less than or equal 0.05). The distributions of Ba and Rb show no significant difference between two groups. The relationships of Fe, Cd and K with HCC were also discussed. The levels of some elements in subcellular fractions of tumor were quite different from those of normal liver, which suggested that trace elements might play important roles in the occurrence and development of hepatocellular carcinoma.

关 键 词：肝癌 亚细胞组分 微量元素 分子活化分析 

分 类 号：O657.4[理学—分析化学] Q581[理学—化学]