来源于羊痘病毒的白介素-18结合蛋白基因的表达和活性研究  

Expression and Bioactivity of IL18-binding Protein Gene from Sheep Poxvirus

作　　者：刘文强[1] 杨少华[2] 栾婧婧[1] 高运东[2] 仲跻峰[2] 赵宏坤[2] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东省农业科学院奶牛研究中心,济南250100

出　　处：《病毒学报》2005年第4期305-307,共3页Chinese Journal of Virology

基　　金：国家"十五"奶业重大科技专项(No.2002BA518A16);山东省"三○"工程基金(2003-3009)

摘　　要：Some poxvirus genome of vaccinia subgenus encoded IL-18 binding prolein gene,which could block IL-18 activity but no sequence similarity to membrane IL-18 receptors.The sheep poxvirus genome subgenus also had this gene.A pair of primers spanning the ORF of goat IL-18 binding protein gene in sheep poxvirus genome were designed.This gene was cloned and expressed in E.coliwith pGEX-5X-3 plasmid.After being purified,the recombinant fusion protein could inhibit goat PBMC IFN- production induced by IL-18 in goat PBMC.Some poxvirus genome of vaccinia subgenus encoded IL-18 binding prolein gene,which could block IL-18 activity but no sequence similarity to membrane IL-18 receptors.The sheep poxvirus genome subgenus also had this gene.A pair of primers spanning the ORF of goat IL-18 binding protein gene in sheep poxvirus genome were designed.This gene was cloned and expressed in E.coliwith pGEX-5X-3 plasmid.After being purified,the recombinant fusion protein could inhibit goat PBMC IFN- production induced by IL-18 in goat PBMC.

关 键 词：羊痘病毒 白细胞介素-18结合蛋白(IL-18BP) 活性 

分 类 号：S852.65[农业科学—基础兽医学]