烟草曲茎病毒卫星DNA在不同寄主中的分子变异比较  

Comparison of Molecular Variation among the Satellite DNA Molecules Associated with Tobacco Curly Shoot Virus in Different Hosts

作　　者：青玲[1] 周雪平[1] 

机构地区：[1]浙江大学生物技术研究所

出　　处：《病毒学报》2005年第4期308-310,共3页Chinese Journal of Virology

基　　金：国家杰出青年基金(No30125032);国家自然科学基金(No30370927)资助项目

摘　　要：The molecular variation of satellite DNA associated with Tobacco curly shoot virus (TbCSV) was compared by DNA sequencing of progenies of TbCSV DNA in Nicotiana benthamiana and N. glutinosa plants agroinoculated with infectious clones of TbCSV and its associated DNA. Comparisons indicated that TbCSV DNA populations had the higher levels of mutation frequency in N. glutinosa than that in N. benthamiana, and mutations generated in N. benthamiana were found upstream or downstream of C1 ORF whereas those in N. glutinosa were mostly located in A-rich region. No mutation was found in C1 ORF and satellite conserved region of DNA. The biological significance of the mutation accumulation and its role in disease epidemic were discussed.The molecular variation of satellite DNA associated with Tobacco curly shoot virus (TbCSV) was compared by DNA sequencing of progenies of TbCSV DNA in Nicotiana benthamiana and N. glutinosa plants agroinoculated with infectious clones of TbCSV and its associated DNA. Comparisons indicated that TbCSV DNA populations had the higher levels of mutation frequency in N. glutinosa than that in N. benthamiana, and mutations generated in N. benthamiana were found upstream or downstream of C1 ORF whereas those in N. glutinosa were mostly located in A-rich region. No mutation was found in C1 ORF and satellite conserved region of DNA. The biological significance of the mutation accumulation and its role in disease epidemic were discussed.

关 键 词：烟草曲茎病毒 卫星DNA 变异 本氏烟 心叶烟 

分 类 号：S435[农业科学—农业昆虫与害虫防治]