REGULAR EXPRESSION OF DISCOIDIN DOMAIN RECEPTOR 2 IN THE IMPROVED ADJUVANT-INDUCED ANIMAL MODEL FOR RHEUMATOID ARTHRITIS  

作　　者：WeiLi Yuan-qiangZhang Xin-pingLiu Li-boYao LanSun 

机构地区：[1]DepartmentofHistologyandEmbryology [2]DepartmentofBiochemistryandMolecularBiology,theFourthMilitaryUniversity,Xi＇an710032

出　　处：《Chinese Medical Sciences Journal》2005年第2期133-137, ,共5页中国医学科学杂志（英文版）

基　　金：Supported by the 973 key research finance of the state(2002CB 513000-07 ).

摘　　要：Objective To investigate the expression of discoidin domain receptor 2 (DDR2) of fibroblast-like synovial cells in im- proved adjuvant-induced animal (AIA) model for rheumatoid arthritis (RA) and to provide evidence for DDR2’s antagonist use clinically. Methods AIA was modified by administrating 0.1 mL of complete Freund’s adjuvant (CFA, mixed with 5 mg Bacillus Calmette-Guerin vaccine/mL) into rats’ right hind paws and 0.125 mL tumor necrosis factor-α (2 U/mL) into right ankles and subpatellar fatty tissue. The expression of DDR2 in fibroblast-like synovial cells was assessed using immunohistochemistry, immunofluorescence histochemistry, and in situ hybridization methods. Levels of anti-collagen II antibody were measured using enzyme-linked immunosorbent assay. Results Given the terms mentioned above, we found a more practical rat model, apparently decreasing immunization time (average 3-5 days). DDR2 can be detected upon the 15th day of immunization; expression gradually increased with time going on, and reaching a peak 35 days after immunization before gradually decreasing. Serum anti-collagen II antibody showed similar expression patterns as DDR2, but reached peak later than DDR2, about 40 days after immunization. Conclusion Regular expression of DDR2 in animal models infers its important role in the pathological process of RA.

关 键 词：rheumatoid arthritis adjuvant-induced arthritis discoidin domain receptor 2 synovial tissue anti-collagen Ⅱ antibody 

分 类 号：R593.21[医药卫生—内科学]