中耳胆脂瘤上皮中白介素-1α的表达  

The expression of interleukin-1α in middle ear cholesteatoma

作　　者：任晓勇[1] 陈文弦[1] 崔鹏程[1] 张全安[2] 

机构地区：[1]第四军医大学唐都医院耳鼻咽喉科,陕西西安710038 [2]西安交通大学第二医院耳鼻咽喉科,陕西西安710004

出　　处：《中国耳鼻咽喉头颈外科》2005年第6期367-369,共3页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：陕西省科技攻关项目基金资助(2004K16-G5)

摘　　要：目的研究白介素-1α(interleukin-1α,IL-1α)在中耳胆脂瘤上皮的表达,并分析与基质金属蛋白酶-9(metalloproteinase-9,MMP-9)以及增殖细胞核抗原(proliferatingcellnuclearantigen,PCNA)表达的关系。方法采用免疫组织化学SP法检测了IL-1α、MMP-9及PCNA在21例继发性胆脂瘤上皮标本与10例胆脂瘤患者外耳道皮肤表皮中的表达。结果IL-1α、MMP-9及PCNA在胆脂瘤上皮的表达,与外耳道皮肤上皮相比明显增强,差异均具显著性(P<0.01,P<0.01,P<0.01)。IL-1α与MMP-9表达存在正相关性(P<0.05),IL-1α与PCNA表达无显著相关性。结论IL-1α与MMP-9表达密切相关,表明IL-1α可通过上调MMP-9的表达在介导胆脂瘤骨质破坏行为中扮演着重要角色。在体内IL-1α可能不是影响胆脂瘤上皮增殖的主要因素。OBJECTIVE To explore the effects ofinterleukin-1 α (IL-1 α) on matrix metalloproteinase-9(MMP-9) expression and activity in middle earcholesteatoma.METHODS IL-1 α, MMP-9 and themarker of cell proliferation, proliferating cell nuclear an-tigen (PCNA) were determined immunohistochemicallyin the cholesteatomas specimens from 21 cases andexternal auditory canal skin specimens from 10 caseswith middle ear cholesteatomas. RESULTS The ex-pression of IL-1α, MMP-9 and PCNA in cholesteatomaepithelium were increased greately than that in externalauditory canal epithelium respectively (P < 0.01).There was a strong positive correlation between IL-1αand MMP-9 expression, while no significantly statisti-cal correlation existed between IL-1 α and PCNAexpression. CONCLUSION IL-1 α may play an impor-tant role in upregulating the expression of MMP-9 invivo which contributes to the bone destruction in middleear cholesteatoma. IL-1 α may not be a key induciblefactor of epithelial proliferation in middle earcholesteatoma.

关 键 词：中耳胆脂瘤上皮 免疫组织化学SP法 IL-1Α MMP-9 基质金属蛋白酶 增殖细胞核抗原 白介素-1Α 继发性胆脂瘤 PCNA表达 cell 皮肤表皮 破坏行为 上皮增殖 外耳道 相关性 显著性 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]