噪声对豚鼠耳蜗电位及其超微结构的影响  

Effect of noise on cochlear potentials and ultrastructure in guinea pigs

作　　者：孙勍[1] 李兴启[1] 单希征[2] 孙建和[1] 

机构地区：[1]解放军总医院耳鼻咽喉科研究所,北京100853 [2]武警总医院耳鼻咽喉头颈外科,北京100039

出　　处：《中国耳鼻咽喉头颈外科》2005年第6期373-376,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金资助项目(39870797)

摘　　要：目的观察噪声暴露对豚鼠耳蜗电位和超微结构的影响。方法选用健康杂色豚鼠10只,以右耳为噪声暴露耳,以左耳为对照耳,右耳持续给白噪声100dBSPL2小时。于噪声暴露前及噪声暴露后测量右耳耳蜗电位,并于噪声暴露后取噪声暴露耳和对照耳的耳蜗,应用透射电镜进行形态学的观察。结果噪声暴露后耳蜗微音电位幅度下降并且其非线性特点消失,听神经复合动作电位阈值明显升高;内毛细胞及其下方传入神经末梢空化,外毛细胞溶酶体增多、胞浆内出现空泡。结论噪声暴露不仅引起外毛细胞的损伤,还可以引起内毛细胞及传入神经纤维的损伤。OBJECTIVE To observe the effect ofnoise on the cochlear potentials and morphology inguinea pigs. METHODS Ten healthy guinea pigs wereused in this study. The left ears of the guinea pigs wereas control ears, the right ears were exposed to 100dBSPL white noise for 2 hours. The cochlear microphonics(CM) and compound action potential (CAP) were re-corded before and after noise exposure. The morpho-logy of the cochlear was observed by transmission elec-tron microscope. RESULTS The amplitude of CM wasdecreased, the nonlinear properties of CM input/outputfunction became linearity after noise exposure. Theaverage CAP threshold was elevated about 50dBSPL. There were vacuoles in out hair cells (OHCs),inner hair cells (IHCs) and radial auditory dendrites.CONCLUSION The results suggest that both the OHCsand IHCs can be damaged after noise exposure.

关 键 词：耳蜗电位 超微结构 听神经复合动作电位 噪声暴露 传入神经纤维 内毛细胞 细胞溶酶体 暴露后 杂色豚鼠 透射电镜 电位幅度 神经末梢 外毛细胞 右耳 白噪声 形态学 非线性 胞浆内 对照 损伤 

分 类 号：R764[医药卫生—耳鼻咽喉科]