高效液相色谱化学发光测定中药制剂中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷  

Determination of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside in the Traditional Chinese Preparation by High Performance Liquid Chromatography with Chemiluminescence Detection

作　　者：卫洪清[1] 刘二保[1] 任荣芳[1] 赵秀丽[2] 李晓霞[2] 江凤仙[2] 

机构地区：[1]天津师范大学化学与生命科学学院,天津300074 [2]山西师范大学化学与材料科学学院,山西临汾041004

出　　处：《光谱学与光谱分析》2005年第6期844-847,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(20075017);山西省自然科学基金(20021022);天津师范大学校基金资助项目

摘　　要：研究了2 ,3,5 ,4′四羟基二苯乙烯2 O βD 葡萄糖苷在硝酸介质中与高锰酸钾的发光行为和光谱现象,甲醛的存在可使化学发光强度大大增强。由于中药制剂成分复杂,干扰大,文章首次采用反相高效液相色谱化学发光技术,建立了一种测定中药制剂中2 ,3,5 ,4′四羟基二苯乙烯2 O βD 葡萄糖苷含量的新方法。对于不同中药制剂样品中2 ,3,5 ,4′四羟基二苯乙烯2 O βD 葡萄糖苷测定的回收率为10 2 %～10 8%。方法的检出限为11 83μg·mL- 1 ,线性范围为15 75～136 5 μg·mL- 1 ,相对标准偏差为3 4 5 % (Cs=2 1 0 μg·mL- 1 ,n =3)。此法简便、快速,重复性好,结果令人满意。Based on the chemiluminescence reaction of KMnO4-HNO3 - 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside sensitized by HCHO, an analytical method for 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside by RP-HPLC with chemiluminescence detector was developed. The chromatographic conditions were optimized. AN ODS hypersil column (25 mm × 4.0 mm, 125 × 4.0 mm i.d. 5 μm) was used with acetonitrile-0.3% H3PO4 [25:75, (ψ)] as mobile phase, and the flow rate was 0.8 mL · min-1 at the column temperature of 40°C. The linear range of calibration curve was from 15.75 to 136.5 μg · mL-1, the detection limit was 11.83 μg · mL-1 with a concentration of 21 μg · mL-1 of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, and the recovery was 102%-108%. The relative standard deviation was 3.45% (Cs=21.0 μg · mL-1, n=3). The method has been applied to the determination of 2,3,5,4'-tetra-hydroxystilbene-2-O-β-D-glucoside in a few Chinese medicines, and the results were satisfactory. The method was simple, rapid and highly selective.

关 键 词：高效液相色谱 化学发光分析 高锰酸钾 2 3 5 4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 

分 类 号：O657.3[理学—分析化学]