达旦黄-曲通X-100体系共振光散射法测定蛋白质  

Microdetermination of Proteins with Titan Yellow by the Resonance Light Scatter ing Method

作　　者：冯素玲[1,2] 王瑾[1,2] 樊静[1,2] 

机构地区：[1]河南师范大学化学与环境科学学院 [2]河南省高等学校环境科学与工程重点学科开放实验室,河南新乡453002

出　　处：《光谱学与光谱分析》2005年第6期927-929,共3页Spectroscopy and Spectral Analysis

基　　金：河南省自然科学基金(0211020200)资助项目

摘　　要：基于在TritonX 10 0存在下,蛋白质与达旦黄作用使得体系的共振光散射增强,在λ=4 92nm处光散射强度最大,增强作用的强弱与蛋白质的含量成正比,据此建立了共振光散射测定蛋白质的新方法。此方法对牛血清白蛋白的检出限达到17 7ng·mL- 1 ,线性范围为0 0 3～0 9μg·mL- 1 ,用于合成样与人血清样品的分析,取得了令人满意的结果。同时也研究了人血清白蛋白、鸡蛋白蛋白、溶菌酶、胰蛋白酶与达旦黄的作用。A resonance light scattering method for the determination of trace proteins was developed. In the presence of Triton X-100, proteins reacted with Titan yellow to form a combination product, resulting a significant enhancement of resonance light scattering (RLS). The Delta I-RLS was directly proportional to the concentration of protein in the range of 0.03-0.9 mu g.mL(-1), with the detection limit 17.7 ng.mL(-1) for BSA. This method was applied to the determination of the proteins in synthetic and human serum samples, and compared to the CBB method, with satisfactory results.

关 键 词：共振光散射 蛋白质 达旦黄 曲通X-100 

分 类 号：O635.3[理学—高分子化学]