人血浆中氯氮平及去甲氯氮平的固相萃取HPLC测定  

Determination of Clozapine and Norclozapine in Human Plasma by HPLC with Solid Phase Extraction

作　　者：迟丹怡[1] 焦正[1] 李中东[1] 施孝金[1] 钟明康[1] 

机构地区：[1]复旦大学附属华山医院临床药学研究室,上海200040

出　　处：《中国医药工业杂志》2005年第7期417-420,共4页Chinese Journal of Pharmaceuticals

摘　　要：建立了HPLC法测定人血浆中的氯氮平及去甲氯氮平含量。采用C18柱,流动相为甲醇-水-三乙胺-乙酸(600∶400∶4∶2),检测波长254nm,固相萃取法处理血样,地西泮为内标。氯氮平及去甲氯氮平在50～2000ng/ml范围内线性关系良好。平均方法回收率分别为103.1%和102.1%。A HPLC method for determination of clozapine and norclozapine in human plasma was established. A C18column was usesd with the mobile phase of methanol-water-triethylamine- acetic acid (600∶400∶ 4∶2) at the detectionwavelength of 254nm. The blood was extracted with solid phase extraction, using diazepam as internal standard. Thecalibration curves were both linear in the range of 50 - 2000ng/ml. The recoveries of clozapine and norclozapine were 103.1%and 102.1%, respectively.

关 键 词：氯氮平 去甲氯氮平 高效液相色谱 固相萃取 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.71[化学工程—制药化工]