氢醌致V79细胞DNA损伤的体外实验研究  

Study of Hydroquinone Induced DNA Damage in V79 Chinese Hamster Cells in vitro

作　　者：张美荣[1] 周建华[2] 时锡金[2] 

机构地区：[1]徐州医学院全科医学系,江苏徐州221002 [2]苏州大学放射医学与公共卫生学院,江苏苏州215007

出　　处：《工业卫生与职业病》2005年第4期219-222,共4页Industrial Health and Occupational Diseases

基　　金：江苏省社会发展基金资助(BS2001050);江苏省高校自然科学基金资助(01KJB330001)

摘　　要：目的研究苯的主要代谢产物氢醌(HQ)对V79细胞DNA的损伤作用。方法分别用0.15,0.30,0.60,1.20,2.40mmol/L的HQ染毒V79细胞2h,用单细胞凝胶电泳方法检测DNA损伤情况。结果在本研究所用剂量范围内,HQ可引起V79细胞DNA的明显损伤,低剂量组(0.15mmol/L)彗星细胞率为69%,彗星尾长(42.20±3.18)μm,高剂量组(2.40mmol/L)彗星细胞率为91%,彗星尾长(49.05±4.31)μm,但无明显的剂量-效应关系。结论HQ可致V79细胞DNA损伤,产生遗传毒性作用。Objective To explore genotoxic mechanism of major metabolite of benzene,hydroquinone(HQ),induced DNA damage in V79 Chinese hamster cells in vitro. Methods V79 Chinese hamster cells were exposed to hydroquinone at different concentrations of 0.15,0.30,0.60,1.20,and 2.40 mmol/L and incubated for 2 h.DNA damage of the cell was detected by single cell gel electrophoresis(SCGE)assay. Results Hydroquinone could induce significant DNA damage at all group concentrations tested.The rate of cells with tail was 69%,the mean value of tail length was((42.20±3.18)μm) in low dose group(0.15 mmol/L)and the rate of cells with tail was 91%,the mean value of tail length was((49.05±4.31)μm) in high dose group(2.40 mmol/L),but no clear dose-dependent response. Conclusions Exposure to HQ could induce DNA damage with genotoxic effects on V79 Chinese hamster cells.

关 键 词：氢醌 DNA损伤 V79细胞 单细胞凝胶电泳 

分 类 号：R114[医药卫生—卫生毒理学]