PAF对长白猪精子活率及运动速度影响的研究  

Effects of Platelet-Activating Factor on Sperm Living Rate and Moving Speed of Changbai Pig

作　　者：秦爱萍[1] 乔淑丽[1] 

机构地区：[1]山西师范大学生物技术工程学院,临汾041000

出　　处：《吉林农业大学学报》2005年第3期328-330,共3页Journal of Jilin Agricultural University

基　　金：国家自然科学基金资助项目(39770553)

摘　　要：在人工采集的长白猪精液中分别加入0,5,10,25,50,100ng mL的血小板活化因子(PAF),于37℃恒温水浴锅中温育5,15,25,35,45,60min。显微镜下检测精子活率,同时用浊度分析法检测快速运动相精子的平均速度(VRM)和含量(FRM)。结果表明:PAF质量浓度为10ng mL的试验组与对照组相比,温育15～60min,精子活率都显著提高(P<0 05);PAF质量浓度为5ng mL的试验组与对照组相比,温育25～45min,精子活率都显著提高(P<0 05);PAF质量浓度为10,25,50,100ng mL的试验组与对照组相比,有显著提高快速运动相精子运动速度的作用(P<0 05);在试验浓度范围内,试验组与对照组相比,精子含量均无显著差异(P>0 05)。认为一定浓度的PAF可提高长白猪精子的活率和快速运动相精子的运动能力。<Abstrcat> Add 0,5,10,25,50 and 100　ng/mL platelet-activating factor (PAF) into the artificially gathered seminal fluid of Changbai pig and breed them in 37℃ thermostatical water for 5,15,25,35,45 and 60 minutes.Measure sperm living rate under the microscope. Meanwhile measure the average speed and percentage of fast-moving sperm with the turbid degree analytic approaches. The results show: Compared with the control group, in the group of 10　ng/mL PAF, bred for 15～60 minutes, the sperm living rate rises notably (P<0.05); in the group of 5　ng/mL PAF, bred for 25～45 minutes, the sperm living rate rises notably (P<0.05); in the groups of 5, 10, 25 and 50　ng/mL PAF, the moving speed of fast-moving sperm increased notably (P<0.05); within the scale of experimental density, compared with the control group, the sperm percentage has little difference (P>0.05). The result indicates that PAF of certain density can improve the sperm living rate and moving ability of fast-moving sperm of Changbai pig.

关 键 词：血小板活化因子 快速运动相精子含量 快速运动相精子平均速度 长白猪 

分 类 号：S828.1[农业科学—畜牧学]