健脑安对脑缺血再灌注后高糖皮质激素促海马神经细胞凋亡作用的影响  

作　　者：魏翠柏[1] 田金洲[2] 

机构地区：[1]首都医科大学宣武医院博士后流动站 [2]北京中医药大学博士生导师／／国家教育部长江学者

出　　处：《世界急危重病医学杂志》2005年第4期797-798,共2页internationl journal of emergency and critical care medicine

摘　　要：Stroke is one of three important diseases threaten healthy of human being. Ischemia stroke with high mortality and disability rate is accout for 55%-80% of stroke. Cerebral ischemia reperfusion injury is one mainly pathological mechanism of ischemia stroke. The functional disturbance of the network of neuro-endocrine and immune (NEI) induced by cerebral ischemia reperfusion heighten abnormally glucocorticiod (GCS) in hippocampus and plasm ,and aggravate pathological impairment after cerebral ischemia reperfusion. Recently investigation showed that excessive GCS is one of important pathological factors causing neuron apoptosis in major brain regions such as hippocampus related to cognitive function, which is responsible for vascular dementia (VaD) after stroke. At present, the mechanism induced neuron apoptosis by higher GCS is remained unkown. JianNaoAn with function of BuShenHuoXueHuaTan, is one effective compound of Chinese herbal drug on curing VaD. The proceding experimental result has confirmed the function of JianNaoAn down-modulated genes expression of IL-1 ,CRF related to NEI and relieved impairment of nerve cells induced by cerebral ischemia -reperfusion. We investigate the action about excessive GCS causing neurons apoptosis after cerebral ischemia and the influence of JianNaoAn on this pathological machenism through radioimmunoassay (RIA), immunohistochemistry, in situ hybridization and TUNEL experimental techniques. Furthermore the protective function of JianNaoAn on neuron after cerebral ischemia-reperfusion and potentially pharmacological methanism to prevent and cure post-stroke dementia were revealed. At 2,6,12,24 h of different ischemia-reperfusion points, the concentration of cortisiol of the JianNaoAn treated group in plasm and hippocampus decreased significantly compared with the model group (P<0.05). The number of positive cells is reduced significantly compared with the model group by TUNEL staining method, especially in reperfusion 12 h,the distinction in number of positive cell between the

关 键 词：海马神经细胞 脑缺血再灌注 糖皮质激素 凋亡作用 健脑安 后高 

分 类 号：R338[医药卫生—人体生理学] R743.31[医药卫生—基础医学]