旋毛虫Ts43基因克隆和序列分析  

Cloning and sequencing of Ts43 gene of Trichinella spiralis

作　　者：韩化敏[1] 崔晶[1] 王中全[1] 卫海燕[1] 晋雪香[1] 

机构地区：[1]郑州大学基础医学院寄生虫学教研室,郑州450052

出　　处：《郑州大学学报（医学版）》2005年第4期616-618,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目30471450;河南省杰出人才创新基金资助项目03210019008;河南省科技创新人才工程项目2003-112-009

摘　　要：目的:构建编码旋毛虫相对分子质量43 000蛋白基因(Ts43)的原核表达载体,并对其序列进行分析.方法:应用RT-PCR方法从河南省南阳猪源旋毛虫获得旋毛虫基因Ts43,克隆人pGEMEX-1载体中构建重组质粒pGEMEX-Ts43,进行测序、同源性比较及抗原性预测分析.结果:所获基因的核苷酸序列与GenBank所登录的旋毛虫Ts43蛋白基因序列的同源性为99%.抗原性预测分析结果显示,河南省南阳猪源旋毛虫相对分子质量43 000的蛋白分子上存在有4个明显抗原活性的结构域,分别位于距翻译起点第26～28、101～105、185～193、275～295个氨基酸残基的肽链上.结论:成功构建了旋毛虫相对分子质量43 000蛋白抗原基因的原核表达载体.Aim: To clone and sequence the gene (Ts43) encoding an antigen of 43 000 molecular weight (MW) of Trichinella spiralis( T. spiralis)and to compare its sequences with that in GenBank. Methods: Ts43 gene fragment was obtained from Henan swine isolates of T. spiralis using RT-PCR. The recombinant plasmid pGEMEX GAAB2 Ts43 was constructed by coloning Ts43 gene into pGEMEX-1 vector. The DNA sequence of Ts43 gene was determined. The homology and the antigenicity of Ts43 gene was analyzed.Results: It was showed that the nucleotide sequencing of the Ts43 gene fragment had 99% homology with that of the Ts43 gene in GenBank. There were 4 structrucal regions with obvious antigenicity in protein molecules with 43 000 MW of Henan swine isolates of T. spiralis,and the 4 structrucal regions were located in the peptide chain of the amino acid 26~28, 101~105,185~193, and 275~295 from the beginning site of translation. Conclusion: The prokaryotic expression vector of the gene of T. spiralis antigenic protein with 43 000 MW is successfully constructed.

关 键 词：旋毛虫 抗原 基因克隆 

分 类 号：R383.15[医药卫生—医学寄生虫学]