妊娠肝内胆汁淤积症对孕鼠肝脏和胎盘的毒性作用  

Toxic effect of pregnancy intrahepatic cholestasls on liver and placenta in pregnant rats

作　　者：邵勇[1] 姚珍薇[1] 李红霞[1] 丁敏[2] 吴味辛[1] 汪克健[3] 廖晓刚[3] 易永芬 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆市400016 [2]重庆医科大学医学检验系,重庆市400041 [3]重庆医科大学电镜室,重庆市400041 [4]重庆医科大学病理科,重庆市400041

出　　处：《世界华人消化杂志》2005年第12期1404-1407,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金项目;No.20475069~~

摘　　要：目的:探讨孕鼠肝内胆汁淤积症对肝脏和胎盘的毒性作用.方法:应用雌孕激素建立妊娠肝内胆汁淤积大鼠模型,光镜和电镜观察母鼠肝脏和胎盘的病理改变.结果:胆淤组和对照组比较,孕鼠血清丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)、总胆汁酸(TBA)的差异有极显著性(AST:3784±155vs747±158;ALT:7076±220vs847±198;TBA:78.5±4.5vs25.2±3.7;P均<0.01).胆淤组孕鼠肝脏光镜下见部分肝细胞有颗粒样变性和空泡变性,电镜下见肝组织中毛细胆管扩张,毛细胆管及肝细胞内见高电子致密物沉积.胆淤组孕鼠胎盘光镜下见部分滋养细胞颗粒样变性和空泡变性.结论:孕鼠肝内胆汁淤积症时高胆汁酸血症对孕鼠肝细胞和胎盘滋养细胞均有明显的细胞毒性作用.AIM: To investigate the toxic effect of intrahepatic cholestasis of pregnancy (ICP) on the liver and placenta in pregnant rats. METHODS: The animal model of ICP was induced by 17-α-ethinylestradiol and progesterone. Pathohistological changes of the liver and placenta of the pregnant rats were observed under light and electronic microscope. RESULTS: The levels of serum alanine transaminase (ALT), aspartate transaminase (AST), and total bile acid (TBA) were significantly higher in ICP rats than the control (AST: 3 784 ± 155 vs 747 ± 158; ALT: 7 076 ± 220 vs 847 ± 198; TBA: 78.5±4.5 vs 25.2±3.7; P<0.01 for all of the three). Granule- and vacuole-like cells were observed in liver of ICP rats under light microscope and Bile canaliculi were dilated and deposition of substances with high electronic density was found in bile canaliculi and hepatocytes of ICP rats by electronic microscopy. Granule- and vacuole-like changes were also found in some placenta syncy-tiotrophoblast of ICP rats by light microscopy. CONCLUSION: ICP with hyperbileacidemia has marked toxicity to hepatocytes and placenta trophoblasts of pregnant rats.

关 键 词：妊娠肝内胆汁淤积症 孕鼠 肝脏 胎盘 毒性作用 

分 类 号：R714.25[医药卫生—妇产科学]