灰盔累枝虫形态学及表膜下纤维系的研究  

STUDIES ON THE MORPHOLOGY AND INFRACILIATURE OF EPISTYLIS GALEA

作　　者：齐桂兰[1] 施心路[1] 冯伟松[2] 

机构地区：[1]哈尔滨师范大学生命与环境学院,哈尔滨150080 [2]中国科学院水生生物研究所,武汉430072

出　　处：《水生生物学报》2005年第4期444-449,共6页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(No.30170108);浙江省自然科学基金(Y304410);浙江省动物学重点学科;国家淡水与生物技术开放实验室基金(No.2002FB09);黑龙江省教育厅自然科学基金联合资助项目

摘　　要：利用活体观察及蛋白银染色技术对灰盔累枝虫(Epistylisgalea)的形态学及表膜下纤维系进行了研究。该种主要鉴别特征为:活体个员的表膜柔软,虫体充分伸展时呈倒长锥形,体长200—300μm,体宽70—90μm;大核“J”字形,呈纵位。单一伸缩泡,直径21—25μm,背位。纵向纤维细密。口围盘纤维呈网状,与纵向纤维直接相连,并在整体上呈兜网状。与本属其他相近种相比,本种第3咽膜(P3)明显要长,其后1/3超出第1咽膜后继续向下呈弧形弯曲。本文对该种上述特征在分类学上的意义及其与相近种类之间的关系进行了讨论。Epistylis galea Ehrenberg,1831,was collected from an eutrophic freshwater pond near Harbin,China,from April to October in 2002.By living and protargol-impregnated specimens,the morphology,infraciliature and myonemic system was studied. Living observations were carried out using both bright field and differential interference contrast microscopy. Protargol staining was done according to the method modified by Shi & Frankel(1990)to reveal the infraciliature and myonemic system of the orga- nism.Drawings were made with the help of the camera lucida. Epistylis galea,a colony in fresh water which has a colonial stalk with two branches,of which the last branch is shortest and is significant metamerism between each of myofilament after impregnated by protargol. Its whole body is relatively soft and highly transparency;while looks like a cone when it uphold completely. As for life morphology zooids are reasonable stout and relatively constant in shape,length in vivo is 200—300μm,width is 70—90μm,and the widest area are at the peristomial lip(PL)of the organism. The peristomial disc(PD)is significantly elevated when the cell is completely extending. Contractiel vacuole is only one,relatively big at top of the cell. Most of the cell organelles are located at the upper 2/3 of organism,such as the vestibulum(infundibulum or buccal cavity),contractile vacuole(CV),macronucleus(Ma),micronucleus(Mi)and food vacuole(FV);the other 1/3 of cell,however,is spacious and rather highly transparency,there are lots of longitudinal figure streaks. The macronucleus(Ma)is“J”-shape,longitudinal originates the middle of peristomial lip(PL),transverse along the organism extend to aboral wreath of cilia(ACW);while macronucleus by protargol impregnated located at the upper of ACW,the funnel is rather wide. Cytopharynx(Cph),which has been used protargol impregnated,is spindle-shape,originates cell’s fore head and reach constantly ACW. The haplokinety(HK)and the polykinety(PK)are parallel with each other from the peak of cell,making app

关 键 词：缘毛目 淡水纤毛虫 灰盔累枝虫 形态特征 表膜下纤维系 

分 类 号：Q959.117.6[生物学—动物学]