病人血清中登革病毒的分离与鉴定  

Isolation and identification of Dengue virus from patient serum

作　　者：何似[1] 李世清[1] 翁育伟[1] 王灵岚[1] 

机构地区：[1]福建省疾病预防控制中心,福州350001

出　　处：《中国人兽共患病杂志》2005年第7期611-612,共2页Chinese Journal of Zoonoses

摘　　要：目的应用细胞分离法从登革热病人急性期血清中分离登革热病毒并应用免疫荧光法证实病毒的血清型.C6/36细胞长成单层后接种急性期血清,以5～7天为一代,观察细胞病变;感染后的细胞使用型特异性抗体进行免疫荧光实验证实其血清型.在10份急性期病人血清中,有6份标本成功分离出登革热I型病毒,其结果和RT-PCR等实验方法的结果一致,分离率为60%.The Dengus viruses were isolated from the acute stage serum samples of patients with Dengue fever by means of cell culture isolation method,in which C6/36 monolayer cells were inoculated with serum samples and the CPE was observed at a 5-7 days interval. The serotypes of the isolates were determined by immunofluorescence assay with serotype-specific monoclonal antibodies. It was demonstrated that 6 strains of Dengue virus typeI were successfully isolated from 10 acute stage serum samples of patients with a isolation rate of 60%,and this result was accordant with that of RT-PCR.

关 键 词：病人血清 分离与鉴定 登革病毒 C6/36细胞 型特异性抗体 免疫荧光法 登革热病毒 细胞分离法 急性期 细胞病变 Ⅰ型病毒 实验方法 血清型 感染后 PCR 分离率 

分 类 号：R512.8[医药卫生—内科学] R282.71[医药卫生—临床医学]