海南省恶性疟原虫氯喹抗性相关基因pfcrt多态性分析  

STUDY ON THE POLYMORPHISMS OF CHLOROQUINERESISTANT GENE pfcrt OF PLASMODIUM FALCIPARUM ISOLATES FROM HAINAN PROVINCE

作　　者：宋杰[1] 江钢锋[2] 陈沛泉[3] 

机构地区：[1]广东医学院寄生虫学教研室,广东湛江524023 [2]广东药学院寄生虫学教研室 [3]广州中医药大学热带病研究所

出　　处：《中国寄生虫病防治杂志》2005年第3期175-177,共3页Chinese Journal of Parasitic Disease Control

摘　　要：目的了解海南省恶性疟原虫产生氯喹抗性是否与pfcrt基因中的关键性点突变有关。方法对45份采自海南省恶性疟患者血样,采用套式PCR法分别扩增pfcrt基因中含有第76位和220位氨基酸的多态性片段,并对扩增产物进行限制性内切酶酶切分析,观察是否存在突变位点。结果45个样本中,pfcrt基因发生K76T点突变的样本有28个,其中20个是抗性株,8个是敏感株;全部样本的pfcrt基因第220位氨基酸均发生A220S点突变。结论我国海南株恶性疟原虫产生氯喹抗性与发生在pfcrt基因中的K76T点突变有一定的关联。Objective To identify whether point mutations existed in pfcrt genes of Plasmodium falciparum from Hainan Province and their relations with chloroquine resistance. Methods 45 blood samples were collected from falciparum malaria cases in Hainan Province. Nested PCR was used to amplify fragments of pfcrt genes including K76T and A220S mutations. PCR products were digested with different restriction endomucleases to detect whether point mutations existed in pfcrt genes. Results The pfcrt K76T mutation was detected in 28 of 45 isolates, 20 of them were chloroquineresistant and the other 8 were chloroquinesensitive. However, A220S mutation was detected in all isolates tested. Conclusion It was clear that a significant association existed between mutations in pfcrt, especially the K76T mutation, and the chloroquine resistance in P. falciparum isolates from Hainan Province .

关 键 词：疟原虫 恶性 pfcrt基因 氯喹抗性 

分 类 号：R382.31[医药卫生—医学寄生虫学]