白首乌茎叶总黄酮对小鼠急性化学性肝损伤的保护作用  

Protective Effect of Total Flavonoids of Cynanchum auriculatum Royle ex Wight on Actue Chemical-induced Liver Injury in Mice

作　　者：吴立云[1] 仓公熬 颜天华[3] 张朝晖[4] 

机构地区：[1]南京医科大学公共卫生学院,江苏南京210029 [2]江苏省疾病预防与控制中心,江苏南京210009 [3]中国药科大学,江苏南京210009 [4]江苏大学,江苏镇江212001

出　　处：《时珍国医国药》2005年第7期615-616,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的:研究白首乌茎叶总黄酮(TFC)对小鼠急性化学性肝损伤的保护作用。方法:腹腔注射四氯化碳(CCl4)制备小鼠急性肝损伤模型,检测血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)活性、肝脏重量指数;同时测定肝组织中丙二醛(MDA)、一氧化氮(NO)含量和超氧化物歧化酶(SOD)活性。结果:连续7d灌胃给予10,20mg/(kg·d)白首乌茎叶总黄酮,能明显抑制肝损伤小鼠血清ALT和AST活性的升高(P<0.01),减轻增加的肝脏重量指数,显著抑制肝组织中MDA和NO含量的升高及SOD活性的下降(P<0.01)。结论:白首乌茎叶总黄酮对CCl4引起的小鼠急性肝损伤具有保护作用,这种作用与其抗脂质过氧化有关。Objective:To study the protective effect of total flavonoids of Cynanchum auriculatum Royle ex Wight (TFC) on actue chemical-induced liver injury in mice.Methods:A liver injury model was induced by administration of carbon tetrachloride(CCl4) into mice .The activities of serum alanine aminotransferase(ALT) and aspate aminotransferase(AST) and the liver indexes were tested. The contents of malondialdehyde (MDA) and nitric oxide(NO) and the activity of superoxide dismutase (SOD) in hepatical tissues in mice were also mearsured.Results:TFC ( given at 10 mg and 20 mg/(kg·d)for 7 days) remarkably inhibited the activities of serum ALT and AST, and decreased the enlarged liver indexes and MDA,NO levels in hepatical tissues in mice. In addition ,it inhibited the decrease of SOD activities.Conclusion:TFC plays a protective action against liver injury induced by CCl4 in mice through anti-lipid peroxide.

关 键 词：总黄酮 四氯化碳 肝损伤 一氧化氮 丙二醛 

分 类 号：R285.5[医药卫生—中药学]