高压氧抑制TGF-β1改善STZ糖尿病大鼠周围神经病变  

Hyperbaric Oxygenation Improves the Peripheral Neuropathy through Inhibiting TGF-β1 in STZ-induced Diabetic Rats

作　　者：刘芳[1] 周景花[2] 丁巧英[3] 彭名淑[4] 管佩玲[2] 张杰[5] 姚庆姑[1] 

机构地区：[1]复旦大学附属上海市第五人民医院内分泌科 [2]复旦大学附属上海市第五人民医院高压氧室,上海200240 [3]复旦大学附属上海市第五人民医院肌电图室,上海200240 [4]复旦大学附属上海市第五人民医院免疫室,上海200240 [5]复旦大学附属上海市第五人民医院病理科,上海200240

出　　处：《复旦学报（医学版）》2005年第4期455-459,共5页Fudan University Journal of Medical Sciences

基　　金：上海市科委资助项目(014119053)

摘　　要：目的研究高压氧治疗(HBOT)对链脲佐菌素(STZ)糖尿病大鼠外周神经传导速度和血清、局部转化生长因子β1(TGF-β1)的影响。方法STZ糖尿病大鼠造模成功2个月,经电镜证实有神经病变后,随机分为3组:①HBOT组(n=31):用胰岛素控制血糖,每天接受0.15MPa、HBOT1h,连续20d;②糖尿病对照(DM,n=32)组:仅用胰岛素控制血糖;③正常对照(NC,n=20)组:腹腔注射生理盐水的无糖尿病的正常STZ大鼠。分别在疗程10、20d时做肌电图后处死,取股动脉血用EIA法测定血清TGF-β1水平,同时取后肢神经进行苏木精-伊红染色、病理学观察和电镜观察,并用TGF-β1多抗对神经组织做DAB二步法免疫组化染色。结果HBOT10d后,治疗组后肢坐骨神经的传导速度明显高于同期DM组(P<0.05),但低于NC组;HBOT20d后,治疗组后肢胫、腓神经的传导速度明显高于同期DM组(P<0.01);而与NC组间无明显差异(P>0.05);血清TGF-β1水平较DM组明显降低(P<0.05),与NC组间无明显差异。病理观察显示神经组织髓鞘内外的TGF-β1染色明显减少。结论HBOT可改善STZ大鼠糖尿病周围神经病变,其机制可能与抑制TGF-β1生成和其在神经组织表达有关。Purpose In order to investigate whether hyperbaric oxygen therapy (HBOT) could improve the peripheral neuropathy in streptozocin (STZ)-induced diabetic rats and its influence on transforming growth factor-β1 (TGF-β1). Methods Diabetic animal model was induced by injection intraperitonally of STZ,and blood glucose were controlled using injection of regular insulin and protamine zine insulin.After 2 months of diabetes onset,the neuropathy was diagnosed through observing the ultrastructure under electron-microscope,and rats were divided randomized into three groups:① Hyperbaric oxygen treatment (HBOT) group(n=31):inhale oxygen (concentration >97%) in animal chamber 1 hour per day at the pressure of 0.15 MPa for 1 to 20 days.② Diabetic model (DM,n=32) group:diabetic rats diagnosed neurepathy without any treatment.③ Normal control (NC,n=20) group:rats were injected normal solution intraperitonally and with normal blood glucose.At the 10 th and 20 th day of oxygenation,the rats in three groups were killed randomized after measuring the nerve conducting velocity(NCV),and the arterial blood were collected from bilateral femoral artery,and the nerve of hind-limb was taken immediately.The glucose levels of nail vein were measured using glucometer before death,and the serum TGF-β1 levels were measured using EIA method.At the same time,the pathological change of nerve tissue was observed using HE staining,and the TGF-β1 immunostaining in peripheral nerve was observed using DAB two-step method.The ultrastructure of them was also observed under electron-microscope. Results Except 1 rat died of anaethesation,30 rats completed the oxygenation in HBOT group.In DM group, excluded 2 died of hypoglycemic coma,data of 30 rats were collected.After 10 days treatment,the serum TGF-β1 levels in HBOT group decreased slightly but significantly than DC group,and they were still higher than NC group.But no obvious neural pathological change was observed in HBOT group,and the improvement of nerve ultrastructure was discovered,like

关 键 词：糖尿病大鼠 转化生长因子β1(TGF-β1) 抑制 血清TGF-Β1 链脲佐菌素(STZ) 糖尿病周围神经病变 HBOT STZ大鼠 神经传导速度 免疫组化染色 控制血糖 神经组织 高压氧治疗 病理学观察 正常对照 生理盐水 腹腔注射 EIA法 伊红染色 

分 类 号：R587.1[医药卫生—内分泌] R587.102[医药卫生—内科学]