γ-硅酸二钙陶瓷的生物活性和细胞毒性研究  

Study on in vitro Bioactivity and Cytotoxicity of γ-Ca_2SiO_4 Ceramic

作　　者：苟中入[1] 常江[1] 翟万银[1] 林开利[1] 曾毅[1] 

机构地区：[1]中国科学院上海硅酸盐研究所生物材料与组织工程研究中心,上海200050

出　　处：《无机材料学报》2005年第4期914-920,共7页Journal of Inorganic Materials

基　　金：上海市科学技术委员会基金(02CJ14009)

摘　　要：对溶胶-凝胶法合成的硅酸二钙粉体通过冷等静压成型,在1450℃下无压烧结,制备出高纯度的,γ-硅酸二钙(γ-Ca2SiO4)陶瓷,并对γ-Ca2SiO4陶瓷的体外(in vitro)生物活性和细胞毒性进行了研究.实验结果表明,该陶瓷具有优良的生物活性,在模拟体液(SBF)中浸泡72h后陶瓷表面沉积类骨碳酸羟基磷灰石层(CHA);材料溶解释放的钙、硅离子对成纤维细胞无毒副作用,在适当浓度下还能促进细胞增殖;该陶瓷还能支持骨髓间质干细胞(BMSCs) 的贴壁、黏附和铺展.因此,γ-Ca2SiO4陶瓷是一种生物活性优良和无细胞毒性的新型生物活性陶瓷材料.In this study, the in vitro bioactivity and cytotoxicity of gamma-dicalcium silicate (γ-Ca2SiO4) ceramic were evaluated systematically. The surface physicochemical changes of the ceramic soaked in a simulated physiological fluid (Kokubo's SBF) for various periods were determined by means of XRD, FT-IR, SEM and EDS analysis. The results indicated that the ceramic surface transformed to a carbonated hydroxyapatite (CHA) within 72 h and a silica-rich layer occurred between the CHA layer and ceramic matrix after 240 h. In addition, the ionic products of the material dissolution had no cytotoxicity to fibroblasts compared with the negative control. This ceramic supported the bone mesenchymal stem cells (BMSCs) adhesion and spreading, and the BMSCs established close contacts with the ceramics after 24h of culture. This combination of properties indicates that the novel γ-Ca2SiO4 ceramic might be suitable for potential application in the biomedical field, preferentially as materials for bone repair.

关 键 词：硅酸二钙 生物活性 碳酸羟基磷灰石 细胞毒性 生物活性陶瓷 

分 类 号：R318[医药卫生—生物医学工程] TB332[医药卫生—基础医学]