不同小麦品种愈伤组织诱导和再生体系建立  

Callus Induction and Plant Regeneration for Different Elite Wheat (Triticum aestivum L.) Varieties

作　　者：祁永斌[1] 李和平[1] 高春生[1] 姚明镜[1] 廖玉才[1] 

机构地区：[1]华中农业大学分子生物技术实验室,武汉430070

出　　处：《武汉植物学研究》2005年第3期227-232,共6页Journal of Wuhan Botanical Research

基　　金：国家转基因植物专项基金资助;国家自然科学基金(30470929)资助。

摘　　要：为了筛选适合组织培养的小麦基因型,建立一套有效的小麦诱导再生体系,以24个小麦品种的幼胚为研究材料,选用4种诱导培养基和3种分化培养基,研究了影响小麦组织培养的各种因素。结果表明:1培养基之间存在显著差异,MM2培养基的诱导效果最好,平均诱导率为98.5%;M5B培养基的分化效果最佳,平均分化率为39.8%。2不同品种在诱导愈伤和分化再生上都有显著的基因型差异。3愈伤组织诱导率和分化率之间无显著相关性。To set up an efficient plant regeneration system and select wheat genotypes suitable for tissue culture,factors affecting tissue culture were studied with the immature embryos of 24 elite wheat varieties under various conditions including four callus induction media and three differen-(tiation) media.The results indicated that MM2 was the best callus induction medium with an (average) induction frequency of 985%,and M5B was the best differentiation medium with an (average) differentiation frequency of 398% in all media.Callus induction and plant regeneration capacity of different varieties were significantly different.There was no significant correlation (between) callus induction and plant regeneration.

关 键 词：小麦 幼胚 愈伤组织诱导 分化再生 

分 类 号：Q943.1[生物学—植物学]