膜表达的HLA-G1对同种反应性PBMC分泌Th1/Th2型细胞因子的影响  

Effects of human leukocyte antigen-G1 on the Th1/Th2 type cytokines production of allogeneic (PBMC)

作　　者：杨惠军[1] 吴雄文[2] 张彩娥[2] 梁智辉[2] 韩军艳[2] 黄亚非[2] 龚非力[2] 

机构地区：[1]湖南师范大学医学院微生物与免疫学教研室,长沙410006 [2]华中科技大学同济医学院免疫学教研室,武汉430030

出　　处：《免疫学杂志》2005年第4期300-302,共3页Immunological Journal

基　　金：教育部留学科研启动基金;武汉市科委"攻关计划"基金(200116009108-3)资助项目

摘　　要：目的研究人脐静脉内皮细胞系(ECV304)表达的HLA-G1对同种异体外周血单个核细胞(PBMC)Th1/Th2型细胞因子分泌的影响。方法采用脂质体介导的基因转染技术将pcDNA3-HLA-G1转入ECV304,以间接免疫荧光技术在蛋白质水平上检测HLA-G1分子在ECV304上的表达;以表达HLA-G1的ECV304作为刺激细胞,灭活后,与健康人PBMC共同培养,用ELISA检测上清液中Th1/Th2型细胞因子的浓度,观察HLA-G1对同种异体抗原激活的PBMC分泌Th1/Th2型细胞因子的影响。结果与转染pcDNA3空质粒的对照组相比,HLA-G1能使PBMC的IL-10分泌增加(P<0.05),而IL-2,IFN-γI、L-4分泌无明显影响。结论HLA-G1能引起由同种异体抗原激活的PBMC分泌IL-10增加,提示HLA-G1有可能使Th1/Th2型细胞因子向Th2型偏移。Objective To evaluate the effects of HLA-G on the Th1/Th2 type cytokines produced by allogeneic peripheral blood monoeuclear cells (PBMCs). Methods The recombinant plasmid pcDNA3-HLA-G1 was transfected into the ECV304 cells using the method of lipofectin transfection, and the expression of membrane-bound HLA-G1 molecule in ECV304 cells was detected by indirect immunofluores-(cence (IIF)). The HLA-G-transfected ECV304 was inactivated, and then co-cultured with normal human PBMCs. The concentration of type cytokines in the supernatants was determined by ELISA. Results The production of IL-10 was significantly increased (P < 0.05) but the production of IL-4, IL-2, and IFN-γ had no significant difference in the supernatants, compared with the controls. Conclusion HLA-G may be one of the factors that can regulate cytokine balance by shifting the Th1/Th2 balance toward Th2 polarization, which is one of important mechanisms of HLA-G in inducing and maintaining immune tolerance to allogeneic antigens.

关 键 词：HLA-G1 ECV304 TH1/TH2型细胞因子 同种移植 免疫耐受 

分 类 号：R392[医药卫生—免疫学]