羊胎盘免疫调节因子E花环活性评价的优化  

Study on biological activity of goat placenta immune-regulating factor by improved E-rosettes method

作　　者：高利臣[1] 钟英英[2] 魏泓[2] 

机构地区：[1]湖南科技大学生命科学学院生物技术系 [2]第三军医大学实验动物中心

出　　处：《免疫学杂志》2005年第4期327-330,共4页Immunological Journal

基　　金：国家自然科学基金资助项目(30070120)

摘　　要：目的改进E花环实验方法,运用改进的实验方法评价羊胎盘免疫调节因子(GPIF)生物活性效果。方法2次将分离的T细胞(猪、山羊胸腺及其外周血和人外周血T细胞)45℃30min灭活,分别加入不同稀释度的GPIF和神经氨酸酶处理的绵羊血红细胞(SRBC),以观察在抑制(病理)条件下,GPIF对T细胞生物活性的影响。结果猪、山羊胸腺及其外周血和人外周血T细胞的E花环实验筛选显示猪胸腺效果最好,其阳性对照组与阴性对照组比差异显著(P<0.05),实验组与阴性对照组比差异极其显著(P<0.01),实验组在0.5mg/mL质量浓度与阴性对照组差异极其显著(P<0.01);使神经氨酸酶处理后的SRBCE花环结环率提高了7%左右。结论经过改进的E花环评价GPIF生物活性具有方法简便、可操作性强、重复性好的特点;实验室研制的GPIF对增强T细胞活性具有剂量效应。Objective To improve E-rosettes method and evaluate biological activity of the goat placenta immune-regulating fac-(tor (GPIF)). Methods The separated T cells were extinguished twice at 45 ℃ for 30 min, to which the GPIF (0.125, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0 mg/mL) and the neuraminidase-treated sheep red blood cells (NSRBC) were added. The effects of GPIF on biological activity of T cells were observed. Re-(sults The) E rosettes formation of the pig thymus T cell was obvious. Significant difference of the E rosettes formation was found between the positive control group and the negative control group (P < 0.05), between the experimental group and the negative control (group (P < 0.05),) as well as between the experimental sample groups and other experimental sample group at 0.5 mg/mL dose (P < 0.01). Conclusion This method is simple, feasible, and repeatable. The GPIF could augment the biological activity of T cells in a dose dependent manner.

关 键 词：羊胎盘免疫调节因子 E花环 T细胞 生物活性 

分 类 号：R392[医药卫生—免疫学]