一个新型钌(Ⅱ)配合物的合成、表征与DNA的键合及溶剂变色性质  

Synthesis, Characterization, DNA-binding and Solvatochromic Properties of a New Ruthenium Complex

作　　者：吴宝燕[1] 高丽华[1] 王科志[2] 

机构地区：[1]北京师范大学化学系,北京100875 [2]北京工商大学环境与化工学院,北京100037

出　　处：《高等学校化学学报》2005年第7期1206-1209,M003,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20371008;90401007);北京市自然科学基金(批准号:2052008)资助

摘　　要：合成和表征了1个新的钌(Ⅱ)配合物[R u(bpy)2(dpapz)](C lO4)2,其中bpy=2,2′-联吡啶,dpapz=联吡啶并[3,2-a:2,′3-′c]-6-氮杂-吩嗪.通过紫外可见光谱、荧光光谱、与溴化乙锭的竞争键合实验和粘度测量研究了该配合物与小牛胸腺DNA的键合性质,并研究了该配合物的紫外可见光谱和荧光光谱的溶剂变色性质.结果表明,该配合物是具有键合常数Kb=6.9×105L/m o l(50 mm o l/L N aC l)的DNA嵌入键合试剂和优良的荧光溶剂传感分子.A new ruthenium complex of -(ClO_4)_2, where bpy=2,2′-bipyridine, dpapz=dipyrido_6-aza-phenazine was synthesized, and characterized by microanalyses, IR and -{-}~1H NMR Spectra. The electronic absorption and emission spectral titration with DNA, DNA competitive binding with ethidium bromide, and viscosity measurements were carried out to study the calf thymus DNA binding properties of -(ClO_4)_2. The solvatochromism of the complex was also studied. The results indicate that the Ru(Ⅱ) complex acts as a DNA intercalator with a binding constant of 6.9×10~5 L/mol at 50 mmol/L NaCl, and an emission sensor for solvent molecules.

关 键 词：钌(Ⅱ)配合物 DNA键合 溶剂变色 

分 类 号：O641.3[理学—物理化学]