无胶筛分芯片电泳研究贝类毒素-软骨藻酸与寡聚DNA的相互作用  

Interaction Between Domoic Acid and Double-Stranded Oligodeoxy-nucleotide Studied by Using Microchip-based Non-gel Sieving Electrophoresis

作　　者：周小棉[1] 李大志[1] 沈铮[1] 刘伟 李桂茹[3] 林炳承[1] 

机构地区：[1]中国科学院大连化学物理研究所 [2]大连海关分析测试中心,大连116001 [3]大连医科大学附属第二医院,大连116023

出　　处：《高等学校化学学报》2005年第7期1252-1254,M009,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20275039);国家自然科学基金重点项目(批准号:20035010);国家自然科学基金重大项目(批准号:20299030);中国科学院仪器研制专项基金资助

摘　　要：The interaction between domoic acid(DA) and 18-mer double-stranded DNA(dsDNA) was investigated by using microchip-based non-gel sieving electrophoresis. For the 18-mer dsDNA with a sequence of 3′-GCATTGGTTGACGTTGCA-5′, the amount of free dsDNA decreases with the increase of the added DA and also with the increase of reactive time. Meanwhile, the newly-produced two peaks were observed. The experimental results show that there is a strong interaction between DA and the 18-mer dsDNA. DA makes the degradation of the 18-mer dsDNA. The non-gel sieving electrophoresis on microchip is a rapid, convenient, highly sensitive method for studying the interaction between DNA and small molecules.The interaction between domoic acid(DA) and 18-mer double-stranded DNA(dsDNA) was investigated by using microchip-based non-gel sieving electrophoresis. For the 18-mer dsDNA with a sequence of 3′-GCATTGGTTGACGTTGCA-5′, the amount of free dsDNA decreases with the increase of the added DA and also with the increase of reactive time. Meanwhile, the newly-produced two peaks were observed. The experimental results show that there is a strong interaction between DA and the 18-mer dsDNA. DA makes the degradation of the 18-mer dsDNA. The non-gel sieving electrophoresis on microchip is a rapid, convenient, highly sensitive method for studying the interaction between DNA and small molecules.

关 键 词：无胶筛分芯片电泳 相互作用 软骨藻酸 双链DNA 

分 类 号：O657[理学—分析化学]