Epithelium-specific ets transcription factor 2 upregulates cytokeratin 18 expression in pulmonary epithelial cells through an interaction with cytokeratin 18 intron 1  

作　　者：Deanna YANIW Jim HU 

机构地区：[1]Programme in Lung Biology Research and the Canadian Institutes of Health Research Group in Lung Development,Hospital for Sick Children,Toronto,Canada M5G 1X8 [2]Institute of Medical Science,University of Toronto,Toronto,Canada M5S 1A1 [3]Departments of Paediatrics,University of Toronto,Toronto,Canada M5S 1A1 [4]Laboratory Medicine and Pathbiology,University of Toronto,Toronto,Canada M5S 1A1.

出　　处：《Cell Research》2005年第6期423-429,共7页细胞研究(英文版)

基　　金：This work was supported by operating grants from the Canadian Institutes of Health Research;the Canadian Cystic Fibrosis Foundation(to J.H.);the Foundation Fighting Blindness—Canada(to J.H.).

摘　　要：The role of Ese-2,an Ets family transcription factor,in gene regulation is not known.In this study,the interactionbetween Ese-2 and cytokeratin 18(K18)intron 1 was characterized in lung epithelial cells.Reporter gene assays showedEse-2 was able to upregulate K18 intron 1 enhanced reporter gene expression by approximately 2-fold.We found thatfull length Ese-2 did not bind DNA strongly,therefore truncated versions of the protein,containing the ETS domain orPointed domain,were created and tested in electrophoresis mobility shift assays.Multiple interactions between the ETSdomain and putative DNA binding sites within K18 intron 1 were observed,which led to the determination of a possibleEse-2 DNA binding consensus sequence.These experiments suggest that Ese-2 could play a role in the regulation ofK18 expression in lung epithelial cells.

关 键 词：transcription factor epithelial cells cytokeratin 18 AIRWAY gene regulation. 

分 类 号：R563[医药卫生—呼吸系统]