鲢的性分化及性转技术的初步研究  

A preliminary study on sex differentiation and sex reversion techniques in Hypophthalmichthys molitrix

作　　者：潘光碧[1] 邹世平[1] 邹桂伟[1] 陈细华[1] 罗相忠[1] 

机构地区：[1]中国水产科学研究院长江水产研究所,湖北荆州434000

出　　处：《上海水产大学学报》2005年第2期127-132,共6页Journal of Shanghai Fisheries University

基　　金：国家"九五"攻关资助项目(96-008-01-02-01)

摘　　要：将繁殖季节顺产出的鲢卵从未受精卵开始到出膜后45d的鱼苗进行连续切片,观察其性分化。经观察,鲢苗出膜后24d在腹腔上皮的下方清晰可见性腺原基。出膜后34d则已明显性分化,雄性生殖嵴之体细胞增殖,与性细胞一起突向体腔,以柄状精巢系膜连于腹膜上皮;雌性生殖嵴之体细胞增殖,沿腹膜上皮横向伸展。用口服甲基睾丸酮(MT)的方法,设3个梯度(15×10-6、30×10-6、45×10-6)对鲢雌核发育苗进行性转,结果以出膜后3～4d的鱼苗口服MT(30×10-6),连续投喂50d,转雄效果较好,鱼苗能正常摄食药饵,成活率可达79.2%。<Abstrcat>Continuous slice observation has been made from unfertilized egg to fry of 34 days after hatching in Hypophthalmichthys molitrix. At the age of 24 days after hatching, the primordial gonad was obviously seen below the peritoneal epithetium. The sex differentiation was completed at the age of 34 days. In males, the genital fold containing the proliferating somatic cells, together with the genital cells, protruded into the cavity and was connected with the peritoneal epithetium by a mesorchium. In females, the genital fold with its somatic cells proliferating, extended along the peritoneal epithetium. Exogenous 17á-Methyltesterone(MT)treatments by oral administration was tried to induce sex reversion in gynogenetic silver carp (Hypophthalmichthys molitrix) fingerlings. with three concentrations(15×10^(-6),30×10^(-6),45×10^(-6)). The results showed that oral administration for continuous 50 days at the level of 30×10^(-6) was effective for sex reversion(from female to male), and in this treatment, the fish could eat the drug-diet and the survival rate was 79.2%, and that soaking and implantation treatments gained poor effect.

关 键 词：鲢 性分化 性转 口服 甲基睾丸酮 

分 类 号：S917[农业科学—水产科学]