小肠黏膜下层与聚丙烯修复大鼠腹壁缺损的对比研究  

ELEMENTARY EVALUATION OF SMALL INTESTINAL SUBMUCOSA AND POLYPROPYLENE MESH USED FOR REPAIRING ABDOMINAL WALL DEFECT IN RATS

作　　者：张喜海[1] 罗静聪[1] 杨志明[1] 项舟[1] 李秀群[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室.干细胞与组织工程研究室,成都610041

出　　处：《中国修复重建外科杂志》2005年第7期574-577,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家高技术研究发展计划(863)资助项目(2002AA205011);国家自然科学基金资助项目(30271317)~~

摘　　要：目的比较小肠黏膜下层(smallintestinalsubmucosa,SIS)和聚丙烯补片(polypropylenemesh,PPM)修复大鼠腹壁缺损的效果,探讨SIS用于腹壁缺损修复的可行性。方法SD大鼠100只,雌雄各半,体重200～250g。手术造成2cm×2cm全层腹壁缺损,随机分为两组(n=50),分别采用相同面积的SIS和PPM修补。术后1、2、4、8和12周分批取材(每时间点10只),行动物一般情况观察、腹壁抗张强度测定、腹腔内粘连情况评价及组织学观察。结果术后动物成活95只,均健康。两种材料未导致动物不良反应,无疝瘘发生,缺损得到完整修复。术后1周,SIS组的腹壁抗张强度值为204.30±5.13mmHg(1mmHg=0.133kPa),PPM组为240.0±10.0mmHg,差异有统计学意义(P<0.05);术后2、4周两组差异无统计学意义;8、12周两组在300mmHg的腹腔压力下均未发生破裂。术后各期SIS组的腹腔粘连程度及强度明显低于同时间点的PPM组,差异有统计学意义(P<0.05)。组织学观察两组均未见明显免疫排斥反应,最终均为纤维结缔组织修复。结论SIS和PPM均能有效修复大鼠2cm×2cm腹壁全层缺损。SIS在生物相容性和抗腹腔粘连方面优于PPM,值得进一步研究。Objective To compare the effect of small intestinal submucosa(SIS) and polypropylene mesh(PPM) on repairing abdominal wall defects in rats, and to probe into the feasibility of using SIS to repair the abdominal wall defects. Methods 100 SD rats(50 males and 50 females)were randomly divided into 2 groups(n=50). Their weight ranged from 200 to (250 g.)Full thickness abdominal wall defects (2 cm×2 cm) were created by surgery and were repaired with SIS and PPM respectively. At different postoperative time (1st, 2nd, 4th, 8th and 12th week), animals were sacrificed to make histological observation. The tensile strength and the development of adhesions were measured and observed. Results 95 animals survived and were healthy after surgery. No inflammatory response and obvious immunoreaction were observed in both groups. One week after operation, the tensile strength of abdominal wall in SIS group (204.30±5.13 mmHg) was lower than that in PPM group(240.0±10.0 mmHg) at 1st week((P<0.05),) and there were no difference at 4th, 8th, 12th week. Adhesions were more marked in PPM group than that in SIS group((P<0.05).) Conclusion Both SIS and PPM are histologically compatible when used in rats and can maintain sufficient tensile strength. SIS is superior to PPM in regards to tissue compatibility and adhesion formation.

关 键 词：腹壁缺损 小肠黏膜下层 聚丙烯 修复 比较 

分 类 号：R656.3[医药卫生—外科学]