精液冷冻对不育患者精子核DNA链完整性影响的研究  

Effect of cryopreservation on sperm DNA integrity of infertile men

作　　者：肖清明[1] 徐红[1] 何宗明[1] 高希海[1] 王新生[2] 孟凡会[2] 

机构地区：[1]滨州职业学院,山东滨州256624 [2]青岛大学医学院附属医院

出　　处：《山东医药》2005年第19期10-11,共2页Shandong Medical Journal

基　　金：山东省医药卫生科技发展计划课题(2003HW001)

摘　　要：目的探讨冷冻保存对不育患者精子核DNA链完整性的影响。方法采用彗星试验检测40例非少精子症不育患者精液冷冻前及复苏后精子核DNA链的完整性。结果复苏后精子彗星细胞率为(41.9±10.7)%,与冷冻前[(40.8±11.2)%]比较差异无显著性(P>0.05);复苏后精子DNA四级损伤彗星细胞率为(20.0±7.0)%,与冷冻前[(11.0±3.4)%]比较差异有非常显著性(P<0.01)。结论精液冷冻对不育患者完整的精子核DNA链无影响,但可能加重冷冻前已有损伤精子核DNA链的损伤程度。Objective:To explore the effects of cryopreservation on sperm DNA integrity of infertile men.Method:Sperm DNA integrity of 40 semen samples from nonoligospermatism infertile men were detected by the using of comet assay before freezing and after thawing.Results:The percentage of comet cells after thawing was (41.9±10.7)%,there was no significant difference compared with that before freezing [(40.8±11.2)%],P>0.05.The percentage of grade four DNA damaged comet cells was significantly increased after thawing [(20.0±7.0)% vs (11.0±3.4)%,P<0.01].Conclusion:Cryopreservation does not affect DNA integrity of undamaged sperm of infertile men,however,it can increase the degree of DNA damage of sperm that had been damaged before freezing.

关 键 词：精子DNA DNA损伤 彗星试验 冷冻保存 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]