小脑颗粒神经元细胞模型材料制备  

Material Preparation of Cerebellar Granule Neurons Cell Model

作　　者：宁章勇[1] 赵德明[1] 杨建民[1] 崔亚利[1] 郝永新[1] 秦秀慧[1] 马李颖[1] 

机构地区：[1]中国农业大学国家动物传染性海绵状脑病实验室,北京100094

出　　处：《中国兽医学报》2005年第4期403-405,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30371062)

摘　　要：小脑颗粒神经元是动物传染性海绵状脑病发生、发展过程中的主要反应性细胞之一,小脑颗粒神经元的体外分离、纯化和鉴定是建立动物传染性海绵状脑病细胞作用模型的关键步骤。根据大鼠小脑颗粒神经元的生存特性,按照抑制筛选的原理,对小脑颗粒神经元进行了体外分离和培养,经神经元特异性的烯醇化酶(NSE)免疫组织化学染色,表明体外培养的小脑颗粒神经元细胞纯度较高,可以用于细胞作用模型的建立。Cerebellar grannule neuron is one of crucial reactive cells in the occurrence and development in animal transmissible spongiform encephalitis(TSE).Culture in vitro,purified and identification of cerebellar grannule neurons is an important procedure in establishing the cell model for transmissible spongiform encephalitis.Based on the different properties of cellular growth pattern,using the measure of controllability filtration,cerebellar grannule neurons were cultured and purified.Immunostaining by symbolic enzyme NSE of neurons,cerebellar grannule neuron were identified and the purification of cell culture is high.Cultured astrocytes can be used for establishing the cell model.

关 键 词：小脑颗粒神经元 培养 鉴定 

分 类 号：S852.33[农业科学—基础兽医学]