gp120mAb对gp120抑制大鼠海马脑片CA_1区LTP作用的研究  

Effect of gp120mAb on long-term potentiation inhibited by HIV-1 gp120 in rat hippocampus in vitro

作　　者：董军[1] 江振友[2] 

机构地区：[1]暨南大学医学院病理生理教研室,广州510632 [2]暨南大学医学院微生物和免疫学教研室,广州510632

出　　处：《中国免疫学杂志》2005年第5期355-358,共4页Chinese Journal of Immunology

基　　金：广东省医学科研基金项目 (A2 0 0 43 2 7);广东省自然科学基金项目 ( 0 40 10 44 3 );广东省中医药管理局项目 ( 10 40 0 74)资助

摘　　要：目的:探讨人类免疫缺陷病毒Ⅰ型(HIV 1)的包膜糖蛋白gp12 0特异抗体gp12 0mAb对gp12 0引起大鼠海马脑片CA1区的突触传递及可塑性变化的影响。方法:应用离体脑片记录技术,记录大鼠海马CA1区的兴奋性突触后电位(EPSP) ,研究gp12 0mAb对gp12 0抑制高频电刺激Schaffer侧支引起的鼠海马长时程增强效应(LTP)作用的影响。结果:gp12 0对高频电刺激(HFS ,10 0Hz ,10 0 0ms×2 ,串间隔2 0秒,共2次)Schaffer侧支引起的大鼠海马CA1区LTP产生抑制作用,而对其基础EPSP没有影响。用浓度为2 0 0pmol/L的gp12 0灌流脑片,可引起LTP的维持发生抑制。这种抑制作用可被gp12 0特异抗体gp12 0mAb( 5 0ng/ml)所拮抗。结论:gp12 0mAb可能是通过拮抗gp12 0抑制海马CA1区的LTP诱发和维持而参与艾滋病痴呆(HIV 1associateddementia ,HAD)Objective:To explor the effect of gp120mAb on synaptic transmission and plasticity changed by gp120 in the CA 1 region of rat hippocampal slices.Methods:We recorded the excitatory postsynaptic potentials (EPSP) and investigated effect of gp120mAb on long-term potentiation inhibited by gp120 in rat hippocampus in vitro.Results:The results showed that HIV-1 gp120 can inhibit the LTP induced by high-frequence stimulation schaffer in CA 1,gp120 had no effect on basal synaptic transmission.HIV-1 gp120(200 pmol/L) can inhibit the LTP maintance,gp120mAb of gp120-specific antibodies can reverse the inhibition effect,But IgG can't reverse it.Conclusion:The reverse effect of gp120-specific antibody gp120mAb on long-term potentiation inhibited by HIV-1 gp120 may contribute to the HAD pathogenesis.

关 键 词：人类免疫缺陷病毒Ⅰ型 gp120特异抗体 长时程增强 海马脑片 

分 类 号：R512.91[医药卫生—内科学]