三肽化合物酪丝亮肽抗肿瘤作用及对单核巨噬细胞激活作用机制  

Anti-Tumor Effects of Tripeptide Tyroserleutide and Its Mechanisms by Activating Monocyte-Macrophages

作　　者：邱爽[1] 陆融 赵岚[1] 王松[1] 周春雷[1] 赵茜[1] 李国力[1] 高文远[3] 姚智[1,3] 

机构地区：[1]天津医科大学免疫教研室,天津300070 [2]深圳康哲药业有限公司,深圳518029 [3]天津大学药学系,天津300072

出　　处：《中国肿瘤生物治疗杂志》2005年第2期129-133,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家"863"高技术研究发展计划资助项目(2004AA2z3170)教育部重点项目(03007)

摘　　要：目的:观察三肽化合物酪丝亮肽的抗肿瘤作用,探讨其对单核巨噬细胞的激活作用。方法:观察YSL对人肝癌BEL-7402裸鼠移植瘤的抑制作用;观察YSL对体外培养。BEL-7402细胞体系的抑制作用;观察YSL对PEMφ杀伤肿瘤细胞BEL-7402及B16-F10的影响;观察YSL对PEMφ分泌合成IL-1β,FNF-α和NO等细胞毒效应分子的影响。结果:YSL能显著抑制BEL-7402移植瘤裸鼠的肿瘤生长,给药剂量为160μg/(kg·d)时疗效最显著,抑制率为44.03%;YSL体外对BEL-7402细胞生长有一定的抑制作用,与阴性对照组比较有显著性差异(P<0.05);YSL能增强裸鼠PEMφ对BEL-7402,B16-F10杀伤作用,与生理盐水对照组相比有显著性差异(P<0.05);YSL能增强Balb/c小鼠PEMφ对BEL-7402,B16-F10杀伤作用,与生理盐水对照组相比有显著性差异(P<0.05);YSL能促进小鼠PEMφ分泌合成细胞毒效应分子IL-1β,TNF-α和NO,与生理盐水对照组相比有显著性差异(P<0.05)。结论:YSl能够抑制BEL-7402的增殖,增强单核巨噬细胞的细胞毒功能,促进细胞霉效应分子IL-1β,TNF-α和NO的分泌合成。Objective:To investigate the anti-tumor effects of the tripeptide tyroserleutide (YSL) and to discuss its mechanisms by activating monocyte-macrophages. Methods:To apply human hepatocarcinoma BEL-7402 tumor transplanted in nude mice to examine the anti-tumor effects of YSL. To apply human hepatocarcinoma cell BEL-7402 to investigated the cytotoxicity of YSL against human hepatocarcinoma BEL-7402 cell line in vitro. To explore the activating effects of YSL on the peritoneal macrophage (PEMφ) functions of cytotoxicity against tumor cell lines (BEL-7402,B16-F10) in vitro and to detected the effects of YSL on the content of cytotoxicity effectors IL-1β,TNF-α and NO produced by PEMφ. Results:YSL could inhibit the growth of transplanted tumor BEL-7402 in nude mice, the inhibition rate of 160μg/(kg·d) was 44.03%. The tumoricidal activity of YSL against BEL-7402 cell line in vitro was observed when compared with the control group (P< 0.05).YSL could activated PEMφ of nude mice and markedly enhance cytotoxicity against tumor cel lines (BEL-7402, B16-F10) when compared with the control group (P<0.05). YSL could activated PEMφ of Balb/c mice and marked enhance cytotoxicity against tumor cell lines (BEL-7402, B16-F10) when compared with producing group (P<0. 05).YSL could stimulate the contents of the cytotoxicity effectors of IL-1β,TNF-α and NO produced by PEMφ(P<0.05). Conclusions:YSL had inhibition functions against human hepatocarcinoma BEL-7402. YSL could increase the cytotoxicity of mono-cyte-macrophages and stimulate producing of the contents of the cytotoxicity effector of IL-1β,TNF-α and NO.

关 键 词：酪丝亮肽 人肝癌BEL-7402 巨噬细胞 细胞毒效应分子 

分 类 号：R392.12[医药卫生—免疫学]