金属卟啉类化合物开管毛细管柱的制备与评价  

Preparation and Evaluation of Metal Tetraphenylporphyrin for Open Tubular Capillary Electrochromatography

作　　者：卢小泉[1] 张强[1] 贾正平[2] 王荣[2] 

机构地区：[1]西北师范大学化工学院,兰州730070 [2]兰州军区兰州总医院,兰州730050

出　　处：《分析化学》2005年第7期935-938,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(20275031;20335030);教育部高校青年教师奖励计划;西北师大科技创新工程(KJCXGC01)资助项目

摘　　要：以锌卟啉、铁卟啉、镍卟啉制备了毛细管电色谱开管柱,以L精氨酸、D,L酪氨酸对其进行了评价,金属卟啉能很好地结合在毛细管内壁,柱效最高达5.3×104理论塔板数/m,保留时间的RSD小于3%,在所选条件下,用制得的毛细管柱分离了L精氨酸、D,L酪氨酸、L天门冬酰胺、L天冬氨酸和D,L胱氨酸,结果令人满意。A series of novel open-tubular capillary electrochromatographic column were prepared by coating with tetraphenylporphyrin zinc, tetraphenylporphyrin iron and tetraphenylporphyrin nickel respectively and evaluated by mixtures containing L-arginine and D,L-tyrosine. The results showed that the metal tetraphenylporphyrin was well bonded inner wall of capillary. The efficiency reached up to 5.3 x 10(4). The relative standard deviation(RSD) of migration time was less than 3%. Under the optimum conditions, L-arginine, L-asparagines, D,L-tyrosine, D,L-cystine and L-asparagic acid were separated successfully.

关 键 词：卟啉类化合物 毛细管柱 评价 制备 L-精氨酸 L-酪氨酸 毛细管电色谱 L-天冬氨酸 理论塔板数 天门冬酰胺 L-胱氨酸 金属卟啉 保留时间 锌卟啉 铁卟啉 开管柱 镍卟啉 管内壁 RsD 柱分离 柱效 

分 类 号：O657.7[理学—分析化学]