毛细管电泳-电化学检测法测定血浆中水溶性小分子抗氧化剂  

Determination of Water-soluble Small Molecular Antioxidants by Capillary Electrophoresis with Electrochemical Detection

作　　者：王清江[1] 陈相[1] 丁飞[1] 吕金[1] 何品刚[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《分析化学》2005年第7期969-971,共3页Chinese Journal of Analytical Chemistry

基　　金：上海市科委自然科学基础研究基金资助项目(No.04ZR14041)

摘　　要：应用毛细管电泳电化学检测方法,以金属铜电极为检测电极,研究了同时测定人血浆中水溶性小分子抗氧化剂谷胱甘肽(GSH)、尿酸(UA)、色氨酸(Try)和半胱氨酸(Cys)的最佳条件。在最佳实验条件下,各组分的线性范围在1.0×10-6～5.0×10-4mol/L内;检出限在10-6mol/L数量级。该法具有分析速度快、灵敏度高等优点,对血浆样品的测定取得了满意的结果。The water-soluble small molecular antioxidants including glutathione (GSH), uric acid (UA), tryptophan ( Try) and eystein (Cys) are very important to human being health and their detection is significant too. Because they are electro-active, electrochemical detection is more effective than ultraviolet detection in their analysis and performs a higher sensitivity. As a powerful separation technique, capillary electrophoresis has some advantages such as cheap instrumentation, low sampling volume and high speed and has been extensive studied and applied in biological analysis in past twenty years. The optimum conditions of determining GSH, UA, Try and Cys by capillary zone electrophoresis with electrochemical detection (CZE-AD) based on metallic copper electrode were studied in detail. The optimum conditions of CZE-AD were separation voltage 15 kV, running buffer 25 mmol/L Na2B4O7-50 mol/L NaH2PO4 (pH 7.9), kinetic injection time 30 s and detection potential 0.5 V. The linear ranges of the four analytes were (1.0 similar to 5.0) x 10(-6) similar to 5.0 x 10(-4) mol/L and the limits of detection were about 10(-6) mol/L under the optimal experimental conditions. This method performed some advantages in quickness, high sensitivity and simple instrumentation, and satisfactory results were obtained when it was applied to analyzing human plasma samples.

关 键 词：毛细管电泳 抗氧化剂 电化学检测法 小分子 水溶性 最佳实验条件 检测方法 检测电极 谷胱甘肽 同时测定 最佳条件 半胱氨酸 线性范围 分析速度 铜电极 人血浆 色氨酸 数量级 检出限 灵敏度 

分 类 号：R446.1[医药卫生—诊断学]