高效液相色谱法测定血浆中左亚叶酸钙的含量  

Determination of Calcium Folinate in Human Plasma by High Performance Liquid Chromatography

作　　者：邓富良[1] 陈本美[1] 饶均明[2] 周平[1] 邓世林[1] 陈新[1] 余俊龙[3] 

机构地区：[1]中南大学湘雅医学院分析测试中心,长沙410078 [2]中南大学湘雅医院检验科,长沙410008 [3]中南大学湘雅医学院病原生物学系,长沙410078

出　　处：《分析化学》2005年第7期996-998,共3页Chinese Journal of Analytical Chemistry

摘　　要：建立了测定血浆中左亚叶酸钙含量的RPHPLC方法。采用DiamonsilC18色谱柱(150mm×4.6mm,5μm),乙腈50mmol/L磷酸氢二钾/磷酸二氢钾缓冲溶液(10∶90,V/V,用磷酸调pH至4.0)为流动相,用前经0.45μm的过滤膜,超声脱气,流速为1.0mL/min,紫外检测波长为290nm,柱温为35℃,进样量20μL。该方法最低检出限为0.2ng;线性范围为0.05～10mg/L,方法平均回收率在97.0%以上;日内及日间精密度的相对标准偏差分别小于4%和6%。该方法简单,快速,灵敏度高,重复性好。A method for determining calcium folinate in human plasma by reversed phase high performance liquid chromatography was developed. Calcium folinate was successfully separated using a Diamonsil C(18) column (200 mm x4.6 min i. d 5 mu m). Mobile phase consisted of acetonitrile-50 mmol/L dipotassiumhydrogen phosphate and potassiumdihydrogen phosphate buffer( adjusted to pH 4.00 using phosphoric acid) solution ( 10: 90) and was degassed ultrasonically before use. Each component of the mobile phase was filtered through a 0.45 mu m membrane. The column temperature was set at 35 degrees C and the flow rate was 1.0 mL/min. Injection volume was 20 mu L. The peak of calcium folinate was monitored at 290 nm. The detection limit was 0.2 ng. The linear range was 0.05 similar to 10 mg/L. The average recovery was over 97.0%. The RSDs of intra-day and inter-day were less than 4% and 6%, respectively.

关 键 词：高效液相色谱法 亚叶酸钙 血浆 测定 HPLC方法 紫外检测波长 磷酸二氢钾 磷酸氢二钾 最低检出限 平均回收率 缓冲溶液 线性范围 相对标准 钙含量 6mm 色谱柱 C18 流动相 过滤膜 min 进样量 精密度 灵敏度 重复性 柱温 

分 类 号：R96[医药卫生—药理学]