毛细管区带电泳分离西孟坦对映异构体  

Chiral Separation of Simendan Enantiomers by Capillary Zone Electrophoresis

作　　者：鹿秀梅[1] 张丹丹[1] 彭颖[1] 李发美[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《分析化学》2005年第7期1003-1006,共4页Chinese Journal of Analytical Chemistry

摘　　要：以βCD为手性选择剂,采用毛细管区带电泳成功分离了西孟坦对映异构体。考察了背景电解质中硼砂缓冲液的pH和浓度、βCD浓度、有机添加剂甲醇含量及分离电压对手性分离的影响,建立了毛细管电泳分离西孟坦对映异构体的方法。最佳分离条件为:20mmol/L硼砂缓冲液(pH11.0,含12mmol/LβCD)甲醇(50∶50,V/V);分离电压20kV。在此条件下西孟坦对映异构体可达基线分离。在25～50mg/L范围内,迁移时间的重复性(RSD)控制在1.9%之内,峰面积重复性的RSD小于4.0%,本方法可用于西孟坦对映异构体的手性分离和定量分析。The enantiomers of simendan was separated by capillary zone electrophoresis using beta-cyclodextrin (beta-CD) as chiral selector. The influences of the concentration and pH of borate buffer solution, beta-CD concentration, methanol content and separation voltage were investigated. A baseline separation of simendan enantiomers was achieved with background electrolyte of 20 mmol/L borate, buffer ( pH 11.0) containing 12 mmol/L beta-CD-methanol (50:50, V/V). The linear range for simcndan enantiomer was 25 similar to 500 mg/L (0.997). The RSDs of migration time and peak area were 1.9% and 4.0%, respectively. This method can be applied in qualitative and quantitative analysis for simendan enantiomers.

关 键 词：对映异构体 毛细管区带电泳 毛细管电泳分离 Β-CD 手性分离 分离电压 手性选择剂 有机添加剂 甲醇含量 分离条件 基线分离 迁移时间 定量分析 缓冲液 重复性 电解质 RSD 峰面积 浓度 硼砂 

分 类 号：O658.9[理学—分析化学]