薰衣草高频植株再生系统的建立  

ESTABLISHMENT OF A HIGH FREQUENCY PLANTLET REGENERATION SYSTEM FOR LAVANDULA ANGUSTIFOLIA CV. MUNSTEAD

作　　者：许耀祖[1] 王晓军[1] 赵民安[1] 赵海清[1] 刘敏[1] 

机构地区：[1]中国科学院新疆理化技术研究所

出　　处：《西南农业大学学报（自然科学版）》2005年第3期344-349,共6页Journal of Southwest Agricultural University

基　　金：中国科学院西部行政高新技术资助项目(KGCX2-SW-506)

摘　　要：以薰衣草叶片为外植体,建立了薰衣草高频植株再生系统,为薰衣草规模化快繁和遗传转化体系的建立奠定了基础。以6-BA,NAA,IBA,Ce(NO3)2,Na2MoO4及蔗糖为实验因子,芽分化率为测定指标,通过L18(61×36)正交实验,筛选了薰衣草芽分化的最佳组合:MS培养基中含有6-BA2.0mg/L,NAA0.5mg/L,IBA0.15mg/L,Ce(NO3)210mg/L,Na2MoO40.5mg/L及蔗糖15g/L。通过L9(34)正交实验,筛选了1种宜于薰衣草生根的理想培养基,即MS+IAA0.5mg/L+NAA0.1mg/L+蔗糖15g/L+活性炭1.0g/L。A high frequency plant regeneration system for Lavandula angustifolia was established to lay a foundation for massive micropropagation and genetic transformation of the plant. The results of an orthogonal experiment showed that the optimum medium for shoot differentiation was MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L + IBA 0.15 mg/L + Ce(NO_3)_2 10 mg/L + Na_2MoO_4 0.5 mg/L + sucrose 15 g/L and the optimum rooting medium was MS + IAA 0.5 mg/L + NAA 0.1 mg/L + sucrose 15 g/L + active carbon 1.0 g/L.

关 键 词：植株再生系统 薰衣草 高频 Na2MoO4 遗传转化体系 正交实验 MS培养基 6-BA 测定指标 芽分化率 最佳组合 外植体 规模化 NAA IBA 蔗糖 活性炭 筛选 快繁 生根 

分 类 号：S687.1[农业科学—观赏园艺] S573.9[农业科学—园艺学]