华北落叶松冷驯化与非冷驯化mRNA差异显示初步分析  

Primary studies on the mRNA differential performance in Larix principis-rupprechtii during cold acclimation and nonacclimation

作　　者：周怀军[1] 左永忠[1] 李坤霞[1] 王润利[1] 安连荣[1] 李帅英[1] 

机构地区：[1]河北农业大学林学院,河北保定071001

出　　处：《河北农业大学学报》2005年第4期32-34,共3页Journal of Hebei Agricultural University

基　　金：河北省教育厅项目(200113)

摘　　要：以华北落叶松(Larixprincipis-rupprechtii)幼苗为研究材料,采用冷驯化和非冷驯化两个处理分别提取RNA,然后逆转录成cDNA,PCR扩增,6%变性丙烯酰胺测序胶电泳,银染显带。结果表明:华北落叶松冷驯化与非冷驯化基因表达有一定差异,经二次扩增初步确定冷驯化处理的扩增产物中在500bp处有一条抗冻特异带。同时,对冷驯化不表达片段进行了分析。Larix principis-rupprechtii seedlings were treated with cold acclimation and nonacclimtion to extract RNA. After treatment, they were reversed transcription as cDNA, PCR amplificated, 6 percent PAGE, and silver stained. The results showed that differential gene expression occurred between cold acclimtion and nonacclimtion of Larix principis-rupprechtii. They were appraised with double amplification after recycling. One antifreeze band was detected primarily and it was 500 bp. Nonexpression gene fragment of larch during cold acclimtion was also analyzed.

关 键 词：华北落叶松 MRNA差异显示 PCR扩增 抗冻蛋白基因片段 

分 类 号：S791.22[农业科学—林木遗传育种]