金标免疫渗滤法检测斯氏狸殖吸虫病患者血清IgM的评价  

Evaluation on detecting serum antibody IgM in patients with Pagumogonimiasis skrjabini by DIGFA

作　　者：陈文碧[1] 王光西[1] 张锡林[2] 杨兴友[1] 佘俊萍[1] 杨燕[1] 

机构地区：[1]泸州医学院病原生物学教研室,四川泸州646000 [2]第三军医大学基础部病原生物学教研室,重庆400038

出　　处：《四川医学》2005年第7期723-725,共3页Sichuan Medical Journal

基　　金：四川省教育厅科研资助项目(2003A062)

摘　　要：目的为寻求简便、可靠的方法,用于斯氏狸殖吸虫病早期诊断及疗效考核。方法建立检测斯氏狸殖吸虫病患者血清IgM抗体的金标免疫渗滤法(DIGFA),并对53例患者在治疗前和治疗后血清中特异抗体水平的动态变化进行研究。结果斯氏狸殖吸虫病患者经有效药物治疗后3个月、6个月、1年、1.5年连续采血,经DIGFA检测连续观察的结果表明,IgM抗体出现较早,阴转较为明显和迅速,治疗后3个月的阴转率就达47.2%(25/53),1年的阴转率可达86.8%(46/53);而IgG抗体则出现较晚,阴转缓慢,治疗后1.5年的阴转率仅为15.1%(8/53)。结论DIGFA检测斯氏狸殖吸虫病患者血清IgM抗体可作为早期诊断及疗效考核的有效方法。Objective To develope a simple and reliable assay for diagnosis and evaluation of treatment on pagumogonimiasis skrjabini.Methods Dot immunogold filtration assay (DIGFA)has been used to detect the change of specific antibodies in sera from 53 pagumogonimiasis skrjabini patients before and after treated with effective drugs.Results Specific antibodies analysis were carried out with sera of different patients,the sera from pagumogonimiasis skrjabini patients 3,6 months,1,1.5 years after and before treatment showed that antibody IgM was appeared earlier than antibody IgG,and disappeared quickly with sera after treatment.3 months and 1 year after treatment reached 47.2%(25/53) and 86.8%(46/53),respectively.However,the disappear rates of antibody IgG only reached 15.1%(8/53)with sera 1.5 year after treatment.Conclusion Detection of antibody IgM by DIGFA can be an effective way for diagnosis and evaluation of treatment on pagumogonimiasis skrjabini.

关 键 词：斯氏狸殖吸虫病 金标免疫渗滤法 

分 类 号：R446.6[医药卫生—诊断学]