异丙酚对兔主动脉阻断脊髓细胞凋亡的影响  

Effect of propofol on spinal cord apoptosis associated with aortic cross-clamping in rabbits

作　　者：柯齐斌[1] 侯俊[1] 陈春[1] 方为[1] 汤和清[1] 李庆华[1] 孙德海[1] 

机构地区：[1]三峡大学第一临床医学院

出　　处：《中国危重病急救医学》2005年第7期426-429,共4页Chinese Critical Care Medicine

基　　金：湖北省宜昌市科技攻关项目(A0430310)

摘　　要：目的探讨异丙酚对家兔主动脉阻断所致脊髓细胞凋亡的影响及其可能的作用机制。方法24只家兔随机分为假手术组(A组)、缺血再灌注损伤组(B组)和异丙酚组(C组)。B、C组阻断腹主动脉40min,再灌注7d;C组于阻断腹主动脉前10min静脉注射异丙酚5mg/kg,继以微量泵静脉持续输注异丙酚20mg·kg-1·h-1直至松夹。分别测定阻断前10min(C10)、开放即刻(C40)、再灌注60min(R60)及7d(R7d)血清丙二醛(MDA)、超氧化物歧化酶(SOD)值;检测脊髓凋亡神经元及Bax、Bcl2蛋白表达;观察术后后肢神经功能。结果1缺血、及再灌注后B组MDA值明显高于C10值及A组相应时间点值(P<0.05或P<0.01),SOD值变化同MDA变化相反;C组MDA值明显高于C10值(P<0.05),但显著低于B组相应时间点值(P<0.05),较A组差异无显著性。2B组Bax蛋白表达明显高于A组(P<0.05),Bcl2蛋白表达明显低于A组(P<0.01);C组Bax蛋白表达明显低于B组,但高于A组(P<0.01和P<0.05),Bcl2蛋白表达明显高于B组及A组(P均<0.01)。3B组凋亡细胞指数明显多于A组;C组明显少于B组,但高于A组(P<0.01和P<0.05)。4C组瘫痪发生率明显低于B组,其后肢神经功能明显好于B组(P均<0.01)。结论异丙酚能抑制家兔主动脉阻断所致的脊髓细胞凋亡的发生,其机制可能与其抗过氧化反应、抑制Bax蛋白、增强Bcl2蛋白的表达有关。Objective To investigate the effect and mechanism of propofol on the spinal cord apoptosis associated with aortic cross-clamping in rabbits. Methods Twenty-four rabbits were randomly divided into sham operation group (A), ischemia/reperfusion group (B) and propofol group (C). In group B and C, the infrarenal aorta was clamped for 40 minutes followed by 7 days reperfusion. Ten minutes before clamping, group C was given propofol 5 mg/kg intravenously and continued at a rate of 20 mg·kg~ -1·h~ -1 until unclamping. The aorta was not clamped in group A. The plasma concentrations of malondialdehyde(MDA) and superoxide dismutase (SOD) were determined at 10 minutes before clamping(C-10), before unclamping (C40), at 60 minutes(R60) and on the 7 th day (R7 d) unclamping. Apoptotic spinal cord cells and expressions of Bax, Bcl-2 protein were measured by immunohistochemical technique. Results ①The concentrations of MDA after ischemia and reperfusion in group B were increased significantly compared with C-10 and those in group A(P<0.05 or P<0.01), which in group C were significantly lower than those in group B (P<0.05), but not in group A. Changes in SOD activity were opposite to those in MDA contents in various groups. ②The expressions of Bax protein in group B were significantly increased compared with those in group A(P<0.05), while the expression of Bcl-2 protein decreased. In group C, Bax protein expression was markedly lower than those in group B and higher than those in group A (P<0.01 and P<0.05), the expression of Bcl-2 was higher than those in groups B and A(both P<0.01). ③ The number of apoptosis cells in group B was much higher than that in group A, which in group C was much lower than that in group B, but higher than that in group A. ④The ratio of paralysis in group C was significantly lower than that in group B with a high neurologic score (both P<0.01). Conclusion Propofol can reduce the spinal cord apoptosis associated with aortic cross-clamping in rabbits. The possible mechanism is related to the ef

关 键 词：异丙酚 兔 主动脉 脊髓 细胞凋亡 BAX蛋白 BCL-2蛋白 

分 类 号：R965[医药卫生—药理学]