陕西省麻疹野病毒的分离及基因分型  

Isolation and Gene Type Detection of Wild-type Measles Viruses in Shaanxi Province

作　　者：李平[1] 司源[1] 武继守[1] 刘西珍[1] 刘毅[1] 常荣芬[2] 关蓉晖[1] 许文波[3] 

机构地区：[1]陕西省疾病预防控制中心,西安710054 [2]西安市儿童医院,陕西西安710000 [3]中国疾病预防控制中心病毒病预防控制所,北京100050

出　　处：《中国计划免疫》2005年第3期184-186,共3页Chinese Journal of Vaccines and Immunization

基　　金：陕西省自然科学基金资助项目(2000SM61)

摘　　要：目的了解陕西省麻疹病毒流行株的基因型别,探讨控制麻疹策略.方法在2000～2003年采集陕西省麻疹爆发和散发病人的咽喉拭子标本56份,用EB病毒转化的狨猴淋巴母细胞(B95a)分离到麻疹病毒15株.用逆转录-聚合酶链反应(RT-PCR)从7株麻疹病毒中扩增出核蛋白(N)基因羧基端450个核苷酸片段,进行核苷酸序列测定和基因分型.结果7株麻疹病毒均属H1基因型,其中H1a4株,H1b3株,H1a和H1b在450个核苷酸片段中的差异为2%～4%.结论H1a和H1b在陕西省隔年流行,流行优势株的变化可能受本土病毒变异和邻省输入病毒的双重影响.Objective In order to learn gene type detection of measles wild virus and the strategy of controlling measles. Methods Genetic characterization of 15 measles viruses isolated from 56 throat swabs or urine specimens of measles patients using B95_a cell line in Shaanxi Province was studied. During 2000-2003, sequence analysis of 450 nucleotides of COOH-terminal of nucleoprotein (N) genes of 7 isolates indicated that all were members of H_1 genotype, among them H_1a-4,H_1b-3,the H_1a subgroup differed from H_1b by 2.00%-4.00% at the nucleotide level in the COOH-terminal of the N gene. Results H_1a and H_1b is endemic every other year. Conclusion The changing of the spread may be influenced by the native virus raviation to input the dual influence of the virus with the heighbour Province.

关 键 词：麻疹病毒 基因分型 

分 类 号：R373.1[医药卫生—病原生物学]