定量酶联免疫吸附试验在检测麻疹IgG抗体中的应用与比较  

Comparative Study on Application of Quantitative ELISA for Detection of Measles Antibody

作　　者：毛乃颖[1] 蒋小泓[1] 吴立萍 南立娟[3] 许文波[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100050 [2]山西省疾病预防控制中心,太原030012 [3]内蒙古自治区疾病预防控制中心,呼和浩特010020

出　　处：《中国计划免疫》2005年第3期187-189,共3页Chinese Journal of Vaccines and Immunization

摘　　要：目的对定量酶联免疫吸附试验(ELISA)在麻疹病毒IgG抗体检测中的应用进行评价,并与传统的半定量ELISA方法做比较。方法收集2003年的92份山西省和内蒙古自治区健康成人血清,用德国维润公司定量ELISA试剂盒测定麻疹IgG抗体滴度。这些血清已经用半定量方法将抗体滴度分为阴性和1∶200、1∶800、1∶3200、1∶12800四个组。将两种方法测定的结果进行比较。结果定性测定结果分析发现,92份血清标本中,5份阴性,1份可疑,86份阳性,与传统半定量ELISA方法的一致率为96·74%,统计分析两实验结果差异无显著的统计学意义。定量测定结果分析发现,随着抗体滴度的增高,该组血清抗体国际单位(IU)也随之上升,均数也随之增高,抗体滴度分组与抗体IU呈明显的正相关,相关系数(rs)为0·963,P<0·000。可重复性检验证明该试剂的可重复性较好。结论定量ELISA方法在麻疹以及其它病毒血清学研究中的应用具有优势。Objective In order to compare the quantiative ELISA with traditional semi-quantitative ELISA on measles IgG antibody. Methods 92 samples of serum from Shanxi and Inner Mongolia were tested by German Vrion-Serion regent,which had been tested by semi-quantitative method with five grades of negative,1∶200,1∶800,1∶3200 and 1∶12 800. Results Analysis on qualitative results showed that in 92 samples,5 were negative,1 was suspicious,86 were positive,and consistency rate was 96.74% with traditional semi-quantitative ELISA.The difference of two methods had no statistical significance.Analysis on quantitative results showed that along with the dilution ratio increased,the test value and mean of international unit of serum antibody increased. Dilution ratio and international unit were positively relative,and the relative coefficient,r_s,was 0.963(P<0.000).Test of repeatable proved the regent could be well repeated. Conclusion The quantitative ELISA on measles has imperative advantage in the fields of serology studies.

关 键 词：麻疹 IGG抗体 定量酶联免疫吸附试验 

分 类 号：R373.11[医药卫生—病原生物学]