HPLC法同时测定补骨脂药材中6种成分的含量  

Determination of Six Constituents in Fructus Psoraleae by HPLC

作　　者：赵陆华[1] 黄朝瑜[1] 屠颖[1] 梅玲华 刘月庆[2] 

机构地区：[1]中国药科大学分析测试中心,南京210009 [2]汇仁集团有限公司,南昌330052

出　　处：《中国天然药物》2005年第4期242-244,共3页

基　　金：国家"十五"重大科技专项"创新药物和中药现代化"(No.2002AA2Z3214)基金项目~~

摘　　要：目的:建立同时测定补骨脂药材中6种成分含量的方法。方法:采用高效液相色谱法同时分析补骨脂中补骨脂素、异补骨脂素、补骨脂甲素、补骨脂异黄酮、补骨脂定和补骨脂查耳酮6种成分。用AlltechC18(4.6mm×250mm,5μm)色谱柱,1%冰醋酸溶液和乙腈为流动相,采用梯度洗脱,流速1.0ml/min,检测波长245nm。结果:6种成分线性关系良好,r>0.9991,平均加样回收率均大于95.0%,RSD均小于3.0%。结论:该方法适用于补骨脂药材中6种成分的同时测定,可作为药材质量的综合评价方法。AIM:To establish a HPLC method for the determination of six constituents:psoralen,isopsoralen,bavachin,coryline,psoralidin,bavachalcone in Psoralea corylifolia L.. METHOD: The sample was analyzed on alltech C_ 18 (4.6 mm×250 mm,5 μm) column with mobile phase consist of 1% acetic acid and acetonitrile,gradient elute (flow rate:1.0 ml/min); wavelength 245 nm. RESULT: The results indicated good linearity,recovery and repeatability. CONCLUSION: The method can be and detected at the used for the quality analysis of six components in Psoralea Corylifolia L.and its preparation.

关 键 词：补骨脂 补骨脂素 异补骨脂素 补骨脂甲素 补骨脂异黄酮 补骨脂定 补骨脂查耳酮 HPLC 

分 类 号：R284.1[医药卫生—中药学]